Construction of Effective Receptor for Recognition of Avian Influenza H5N1 Protein HA1 by Assembly of Monohead Glycolipids on Polydiacetylene Vesicle Surface
文献类型：期刊论文
| 作者 | Deng, Jieli1; Sheng, Zhonghan1; Zhou, Kai2; Duan, Mingxing2; Yu, Chu-yi1; Jiang, Long1 |
| 刊名 | BIOCONJUGATE CHEMISTRY
 |
| 出版日期 | 2009-03-01 |
| 卷号 | 20期号:3页码:533-537 |
| ISSN号 | 1043-1802 |
| DOI | 10.1021/bc800453u |
| 英文摘要 | Fast and sensitive detection of epidemic virus is of the utmost importance for human being in nowadays. Various biosensors have been designed for this goal based on conjugation event between host cell glycolipids and invading virus. However, multihead glycolipids analogous to native receptors on cell surface are known to be very difficult to mimic because of the complexity of chemical synthesis. Here, we developed a new approach where two types of monohead glycolipids, active sialic acid-beta-glucoside (G1) and inactive lactose-beta-glucoside (G2), are embedded onto the surface of a polydiacetylene (PDA) vesicle to mimic native glycolipids on the cell surface. Vesicles prepared in this manner show good selectivity with a 10 ng/mL detection limit and 5 min response time to Hemagglutinin (HA1), which is more sensitive than any HA1 biosensors ever known. Moreover, in the formation of color-changeable vesicles, a very strong synergistic effect between G1 and G2 has been found, offering a novel strategy to construct effective biosensor receptors, as well as a new way to study the surface combination effect that is potentially important to the immunology study of epidemic disease. |
| 语种 | 英语 |
| WOS记录号 | WOS:000264389800017 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/67523]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jiang, Long |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Jieli,Sheng, Zhonghan,Zhou, Kai,et al. Construction of Effective Receptor for Recognition of Avian Influenza H5N1 Protein HA1 by Assembly of Monohead Glycolipids on Polydiacetylene Vesicle Surface[J]. BIOCONJUGATE CHEMISTRY,2009,20(3):533-537. |
| APA | Deng, Jieli,Sheng, Zhonghan,Zhou, Kai,Duan, Mingxing,Yu, Chu-yi,&Jiang, Long.(2009).Construction of Effective Receptor for Recognition of Avian Influenza H5N1 Protein HA1 by Assembly of Monohead Glycolipids on Polydiacetylene Vesicle Surface.BIOCONJUGATE CHEMISTRY,20(3),533-537. |
| MLA | Deng, Jieli,et al."Construction of Effective Receptor for Recognition of Avian Influenza H5N1 Protein HA1 by Assembly of Monohead Glycolipids on Polydiacetylene Vesicle Surface".BIOCONJUGATE CHEMISTRY 20.3(2009):533-537. |
入库方式： OAI收割
来源：化学研究所
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