Analysis of aristolochic acids by CE-MS with carboxymethyl chitosan-coated capillary
文献类型：期刊论文
| 作者 | Fu, Xiaofang1; Liu, Yi1; Li, Wei1; Pang, Nannan1; Nie, Honggang1; Liu, Huwei1; Cai, Zongwei2 |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2009-05-01 |
| 卷号 | 30期号:10页码:1783-1789 |
| 关键词 | Aristolochic Acid Capillary Coating Carboxymethyl Chitosan Ce Ms |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.200800487 |
| 英文摘要 | A CE-MS method for rapid determination of aristolochic acid-I and aristolochic acid-II (AA-II) in traditional Chinese medicines and biological samples was described in the present paper. AA-I and AA-II can be baseline separated within 6 min by CE-MS with carboxymethyl-chitosan-coated capillary. CZE conditions including pH, concentration of buffer, applied voltage, and capillary temperature were systematically investigated, and the composition and flow rate of sheath liquid were also optimized for CE-MS. Furthermore, the CE-UV method without any additives in BGE solution was established and compared with the CE-MS method. The results showed that the two methods could achieve satisfactory separation efficiency, repeatability, and linearity, while the LOD was 0.6 mu g/mL for CE-UV and 0.05 mu g/mL for CE-MS. Compared with the CE-UV method, the sensitivity of CE-MS was significantly improved, in addition to the structure information provided by MS detection at the same time. As an application example, a spiked sample in human serum was analyzed by the CE-MS method, indicating that the new CE-MS method can be applied to analyze AAs in biological samples. |
| 语种 | 英语 |
| WOS记录号 | WOS:000266524500020 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/67667]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Huwei |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci,Inst Analyt Chem, Key Lab Bioorgan Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China 2.Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Xiaofang,Liu, Yi,Li, Wei,et al. Analysis of aristolochic acids by CE-MS with carboxymethyl chitosan-coated capillary[J]. ELECTROPHORESIS,2009,30(10):1783-1789. |
| APA | Fu, Xiaofang.,Liu, Yi.,Li, Wei.,Pang, Nannan.,Nie, Honggang.,...&Cai, Zongwei.(2009).Analysis of aristolochic acids by CE-MS with carboxymethyl chitosan-coated capillary.ELECTROPHORESIS,30(10),1783-1789. |
| MLA | Fu, Xiaofang,et al."Analysis of aristolochic acids by CE-MS with carboxymethyl chitosan-coated capillary".ELECTROPHORESIS 30.10(2009):1783-1789. |
入库方式： OAI收割
来源：化学研究所
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