Analysis of local polarity change around Cys34 in bovine serum albumin during N -> B transition by a polarity-sensitive fluorescence probe
文献类型：期刊论文
| 作者 | Wang, Xiaochun1,2; Guo, Lihong3; Ma, Huimin1 |
| 刊名 | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 73期号:5页码:875-878 |
| 关键词 | Bovine Serum Albumin n -> b Transition Cys34 Domain Local Polarity Fluorescence Probe |
| ISSN号 | 1386-1425 |
| DOI | 10.1016/j.saa.2009.04.008 |
| 英文摘要 | The change trend of the local environment of Cys34 domain in bovine serum albumin has been studied as a function of pH value by using thiol-specific and polarity-sensitive fluorescent probe 3-(4-chloro-6-p-maleimidylphenoxyl-1,3,5-triazinylamino)-7-dimethylamino-2-methyl-phenazine. The local polarity of the Cys34 domain is found to rise with the increase of pH values, and the corresponding dielectric constant is raised from 12.8 at pH 6.0 to 23.3 at pH 9.1. The result shows that the environment of the Cys34 domain is rather hydrophobic in normal state at pH 6.0 and becomes a little hydrophilic in the course of N -> B transition, which may be attributed to the slight unfolding of the protein and thus the increasing of exposure of the previously relatively buried Cys34. In addition, the increased dielectric constant (23.3) is much lower than that (80.1) of water, suggesting that the unfolding of bovine serum albumin does not cause the full exposure of the Cys34 to the aqueous media during the transition. (C) 2009 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000268009400015 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/67705]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma, Huimin |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Natl Res Ctr Geoanal, Beijing 100037, Peoples R China 3.Beijing Mil Area Command, Joint Logitist Dept, Inst Drug & Instrument Control, Beijing 100071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaochun,Guo, Lihong,Ma, Huimin. Analysis of local polarity change around Cys34 in bovine serum albumin during N -> B transition by a polarity-sensitive fluorescence probe[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2009,73(5):875-878. |
| APA | Wang, Xiaochun,Guo, Lihong,&Ma, Huimin.(2009).Analysis of local polarity change around Cys34 in bovine serum albumin during N -> B transition by a polarity-sensitive fluorescence probe.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,73(5),875-878. |
| MLA | Wang, Xiaochun,et al."Analysis of local polarity change around Cys34 in bovine serum albumin during N -> B transition by a polarity-sensitive fluorescence probe".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 73.5(2009):875-878. |
入库方式： OAI收割
来源：化学研究所
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