Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells
文献类型：期刊论文
| 作者 | Zhao, Zilong1; Xu, Li1; Shi, Xiaoli1; Tan, Weihong2; Fang, Xiaohong1; Shangguan, Dihua1 |
| 刊名 | ANALYST
 |
| 出版日期 | 2009 |
| 卷号 | 134期号:9页码:1808-1814 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/b904476k |
| 英文摘要 | In this work, we have developed new aptamer probes for non-small cell lung cancer (NSCLC) by directing the aptamer selection process against the living cells of adenocarcinoma, the most common subtype of NSCLC. A panel of single-stranded DNA (ssDNA) aptamers were generated and evaluated for adenocarcinoma cell recognition. The aptamers bound to the adenocarcinoma cells with dissociation constants in the nanomolar range and the binding of the selected aptamers to the adenocarcinoma cells were significantly stronger than the other cancerous lung cells as well as other types of cancer cells. Moreover, the application of the aptamers to the clinical tissue section samples showed the differentiation of adenocarcinoma from normal lung tissue and other subtypes of lung cancer. The aptamers are expected to be new molecular probes for the investigation of the molecular bases of different NSCLC subtypes and their biological heterogeneity, which is valuable for advancing NSCLC diagnosis and treatment. |
| 语种 | 英语 |
| WOS记录号 | WOS:000269063100010 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/67827]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, Xiaohong |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Univ Florida, Dept Chem, Shands Canc Ctr, Gainesville, FL 32601 USA |
| 推荐引用方式 GB/T 7714 | Zhao, Zilong,Xu, Li,Shi, Xiaoli,et al. Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells[J]. ANALYST,2009,134(9):1808-1814. |
| APA | Zhao, Zilong,Xu, Li,Shi, Xiaoli,Tan, Weihong,Fang, Xiaohong,&Shangguan, Dihua.(2009).Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells.ANALYST,134(9),1808-1814. |
| MLA | Zhao, Zilong,et al."Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells".ANALYST 134.9(2009):1808-1814. |
入库方式： OAI收割
来源：化学研究所
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