Magnetically assisted fluorescence ratiometric assays for adenosine deaminase using water-soluble conjugated polymers
文献类型：期刊论文
| 作者 | He Fang; Yu MingHui; Wang Shu |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 54期号:8页码:1340-1344 |
| 关键词 | Conjugated Polymer Aptamer Adenosine Deaminase Magnetic Bead |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-009-0195-2 |
| 英文摘要 | A magnetically assisted fluorescence ratiometric technique has been developed for adenosine deaminase assays with high sensitivity using water-soluble cationic conjugated polymers (CCPs). The assay contains three elements: a biotin-labeled aptamer of adenosine (biotin-aptamer), a signaling probe single-stranded DNA-tagged fluorescein at terminus (ssDNA-Fl) and a CCP. The specific binding of adenosine to biotin-aptamer makes biotin-aptamer and ssDNA-Fl unhybridized, and the ssDNA-Fl is washed out after streptavidin-coated magnetic beads are added and separated from the assay solution under magnetic field. In this case, after the addition of CCP to the magnetic beads solution, the fluorescence resonance energy transfer (FRET) from CCP to fluorescein is inefficient. Upon adding adenosine deaminase, the adenosine is converted into inosine, and the biotin-aptamer is hybridized with ssDNA-Fl to form doubled stranded DNA (biotin-dsDNA-Fl). The ssDNA-Fl is attached to the magnetic beads at the separation step, and the addition of CCP to the magnetic beads solution leads to efficient FRET from CCP to fluorescein. Thus the adenosine deaminase activity can be monitored by fluorescence spectra in view of the intensity decrease of CCP emission or the increase of fluorescein emission in aqueous solutions. The assay integrates surface-functionalized magnetic particles with significant amplification of detection signal of water-soluble cationic conjugated polymers. |
| 语种 | 英语 |
| WOS记录号 | WOS:000265019200010 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/67915]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang Shu |
| 作者单位 | Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Organ Solids, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | He Fang,Yu MingHui,Wang Shu. Magnetically assisted fluorescence ratiometric assays for adenosine deaminase using water-soluble conjugated polymers[J]. CHINESE SCIENCE BULLETIN,2009,54(8):1340-1344. |
| APA | He Fang,Yu MingHui,&Wang Shu.(2009).Magnetically assisted fluorescence ratiometric assays for adenosine deaminase using water-soluble conjugated polymers.CHINESE SCIENCE BULLETIN,54(8),1340-1344. |
| MLA | He Fang,et al."Magnetically assisted fluorescence ratiometric assays for adenosine deaminase using water-soluble conjugated polymers".CHINESE SCIENCE BULLETIN 54.8(2009):1340-1344. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。