Water-Soluble Conjugated Polyelectrolyte-Based Fluorescence Enzyme Coupling Protocol for Continuous and Sensitive beta-Galactosidase Detection
文献类型：期刊论文
| 作者 | Feng, Fude; Liu, Libing; Wang, Shu |
| 刊名 | MACROMOLECULAR CHEMISTRY AND PHYSICS
 |
| 出版日期 | 2009-08-06 |
| 卷号 | 210期号:15页码:1188-1193 |
| 关键词 | Enzymes Fluorescence Polyelectrolytes Sensors Synthesis |
| ISSN号 | 1022-1352 |
| DOI | 10.1002/macp.200900264 |
| 英文摘要 | A simple, rapid, continuous and homogeneous fluorescent assay for P-galactosidase was developed, combining an enzyme-coupled reaction and signal amplification property of conjugated polyelectrolytes (CPs). The procedure is based on a sequence of two coupled biocatalytic steps in which the beta-galactosidase hydrolyzes its substrate to a phenol derivative followed by conversion to quinone (secondary product) with fluorescence quenching ability by the tyrosinase. The fluorescence of PFP-SO(3)(-) is efficiently quenched by the quinone via an electron transfer process. The limit of detection (LOD) of this assay is less than 0.0005 U.mL(-1), which is better than that of electrochemical method and is comparable to that of most sensitive chemilumine scent techniques. In principle, this sensor mechanism will extend the application window of Us for wide-spectrum enzyme detections. This "mix-and-detect" approach could be expanded to a high-throughput manner. |
| 语种 | 英语 |
| WOS记录号 | WOS:000269175100001 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/67963]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Libing |
| 作者单位 | Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Fude,Liu, Libing,Wang, Shu. Water-Soluble Conjugated Polyelectrolyte-Based Fluorescence Enzyme Coupling Protocol for Continuous and Sensitive beta-Galactosidase Detection[J]. MACROMOLECULAR CHEMISTRY AND PHYSICS,2009,210(15):1188-1193. |
| APA | Feng, Fude,Liu, Libing,&Wang, Shu.(2009).Water-Soluble Conjugated Polyelectrolyte-Based Fluorescence Enzyme Coupling Protocol for Continuous and Sensitive beta-Galactosidase Detection.MACROMOLECULAR CHEMISTRY AND PHYSICS,210(15),1188-1193. |
| MLA | Feng, Fude,et al."Water-Soluble Conjugated Polyelectrolyte-Based Fluorescence Enzyme Coupling Protocol for Continuous and Sensitive beta-Galactosidase Detection".MACROMOLECULAR CHEMISTRY AND PHYSICS 210.15(2009):1188-1193. |
入库方式： OAI收割
来源：化学研究所
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