Preparation and Bioactivity of the Composite of PLGA and Hydroxyapatite Nanocrystals Surface-grafted with L-lactic Acid Oligomer
文献类型：期刊论文
| 作者 | Yu Ting1,2; Liu Ya1; Wang Yu2; Jing Xia-Bin2; Zhang Pei-Biao2; Chen Xue-Si2 |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2009-07-10 |
| 卷号 | 30期号:7页码:1439-1444 |
| 关键词 | Hydroxyapatite Poly(Lactide-co-glycolide) Surface Grafting Nanocomposite Osteogenic Bioactivity |
| ISSN号 | 0251-0790 |
| 英文摘要 | The hydroxyapatite (HA) nanocrystals of 100-200 nm in length and 20-30 nm in width were hydrothermally synthesized by the reaction of phosphoric acid and calcium hydroxide. Lactic acid oligomer surface grafted HA(op-HA) nanoparticles were obtained by oligomeric lactic acid with a certain molecular weight grafting onto the HA surface to form a Ca carboxylate bond in the absence of any catalyst. The op-HA was further blended with poly(lactide-co-glycolide) (PLGA) to prepare the nanocomposite of op-HA/PLGA. FTIR, TGA, ESEM and EDX were used to analyze grafting reaction, the graft ratio of op-HA, surface topography and calcium deposition of the composites, respectively. The rabbit osteoblasts were seeded and cultured on the surface of composites in vitro. The cell morphology, adhesion, proliferation and gene expression were evaluated with FITC staining, NIH image J software and the analysis of real-time PCR, respectively. The results show that the graft ratio of op-HA is 8.3% (mass fraction). The op-HA/PLGA nanocomposite possessed more suitable surface properties, including roughness and plenty of calcium and phosphor. It exhibited better cell adhesion, spreading and proliferation of rabbit osteoblasts, compared to pure PLGA. Its gene expression of Collagen-I, Bone Morphogenetic Protein 2 (BMP-2) and Osteonectin were higher than that of PLGA. It indicated that the blocompatibility and osteogenic bioactivity of the op-HA/PLGA nanocomposite were improved obviously than that of the pure PLGA. |
| 语种 | 英语 |
| WOS记录号 | WOS:000268467100038 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68191]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu Ya |
| 作者单位 | 1.Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu Ting,Liu Ya,Wang Yu,et al. Preparation and Bioactivity of the Composite of PLGA and Hydroxyapatite Nanocrystals Surface-grafted with L-lactic Acid Oligomer[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2009,30(7):1439-1444. |
| APA | Yu Ting,Liu Ya,Wang Yu,Jing Xia-Bin,Zhang Pei-Biao,&Chen Xue-Si.(2009).Preparation and Bioactivity of the Composite of PLGA and Hydroxyapatite Nanocrystals Surface-grafted with L-lactic Acid Oligomer.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,30(7),1439-1444. |
| MLA | Yu Ting,et al."Preparation and Bioactivity of the Composite of PLGA and Hydroxyapatite Nanocrystals Surface-grafted with L-lactic Acid Oligomer".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 30.7(2009):1439-1444. |
入库方式： OAI收割
来源：化学研究所
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