Analysis of local structure of Arg10 domain in apo-alpha-lactalbumin with a polarity-sensitive arginine-specific fluorescent probe
文献类型：期刊论文
| 作者 | Wang ShuJuan; Chen SuMing; Ma HuiMin |
| 刊名 | SCIENCE IN CHINA SERIES B-CHEMISTRY
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 52期号:6页码:809-814 |
| 关键词 | Arginine-specific Labeling Fluorescent Probes Local Polarity Detection Conformational Changes Ca(2+) Binding |
| ISSN号 | 1006-9291 |
| DOI | 10.1007/s11426-009-0027-x |
| 英文摘要 | The polarity-sensitive fluorescent probe, 3-(4-chloro-6-p-glyoxalphenoxy-1,3,5-triazinylamino)-7-(dimethylamino)-2-methylphenazine, was used to analyze the local structure of apo-alpha-lactalbumin by detecting the polarity and conformational changes of the arginine residue (Arg10) domain. The polarity of the Arg10 domain in both native and heat-denatured apo-alpha-lactalbumin was determined, which corresponds to a dielectric constant of 16, and the hydrophobic core near the Arg10 was found to be conservative for heating. Meanwhile, the effect of Ca(2+) binding on the conformational changes of the Arg10 domain was studied, revealing that the hydrophobic core near the Arg10 is insensitive to the binding of Ca(2+). |
| 语种 | 英语 |
| WOS记录号 | WOS:000266168000017 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68231]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma HuiMin |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang ShuJuan,Chen SuMing,Ma HuiMin. Analysis of local structure of Arg10 domain in apo-alpha-lactalbumin with a polarity-sensitive arginine-specific fluorescent probe[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(6):809-814. |
| APA | Wang ShuJuan,Chen SuMing,&Ma HuiMin.(2009).Analysis of local structure of Arg10 domain in apo-alpha-lactalbumin with a polarity-sensitive arginine-specific fluorescent probe.SCIENCE IN CHINA SERIES B-CHEMISTRY,52(6),809-814. |
| MLA | Wang ShuJuan,et al."Analysis of local structure of Arg10 domain in apo-alpha-lactalbumin with a polarity-sensitive arginine-specific fluorescent probe".SCIENCE IN CHINA SERIES B-CHEMISTRY 52.6(2009):809-814. |
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来源：化学研究所
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