Cationic conjugated polymers for homogeneous and sensitive fluorescence detection of hyaluronidase
文献类型：期刊论文
| 作者 | An LingLing; Liu LiBing; Wang Shu1 |
| 刊名 | SCIENCE IN CHINA SERIES B-CHEMISTRY
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 52期号:6页码:827-832 |
| 关键词 | Conjugated Polyelectrolytes Energy Transfer Biosensors Hyaluronidase |
| ISSN号 | 1006-9291 |
| DOI | 10.1007/s11426-009-0073-4 |
| 英文摘要 | The cationic charged water-soluble polyfluorenes containing 2,1,3-benzothiadiazole (BT) units (P1-3) have been synthesized and characterized. These polymers demonstrate intramolecular energy transfer from the fluorene units to the BT sites when oppositely charged hyaluronan is added due to the formation of electrostatic complexes, followed by a shift in emission color from blue to green or brown. Upon adding hyaluronidase, the hyaluronan is cleaved into fragments. The relatively weak electrostatic interactions of hyaluronan fragments with polyfluorenes keep their main chains separated and energy transfer from the fluorene units to the BT sites is inefficient, and the polyfluorenes recover their blue emissions. The complexes of conjugated polymers/hyaluronan can be utilized as probes for sensitive and facile fluorescence assays for hyaluronidase. The new assay method interfaces with the aggregation and light harvesting properties of conjugated polymers. |
| 语种 | 英语 |
| WOS记录号 | WOS:000266168000020 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68255]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang Shu |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | An LingLing,Liu LiBing,Wang Shu. Cationic conjugated polymers for homogeneous and sensitive fluorescence detection of hyaluronidase[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(6):827-832. |
| APA | An LingLing,Liu LiBing,&Wang Shu.(2009).Cationic conjugated polymers for homogeneous and sensitive fluorescence detection of hyaluronidase.SCIENCE IN CHINA SERIES B-CHEMISTRY,52(6),827-832. |
| MLA | An LingLing,et al."Cationic conjugated polymers for homogeneous and sensitive fluorescence detection of hyaluronidase".SCIENCE IN CHINA SERIES B-CHEMISTRY 52.6(2009):827-832. |
入库方式： OAI收割
来源：化学研究所
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