Determination of monoamine neurotransmitters and their metabolites in a mouse brain microdialysate by coupling high-performance liquid chromatography with gold nanoparticle-initiated chemiluminescence
文献类型：期刊论文
| 作者 | Li, Na1; Guo, Jizhao1; Liu, Bo1; Yu, Yuqi1; Cui, Hua1; Mao, Lanqun2; Lin, Yuqing2 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2009-07-10 |
| 卷号 | 645期号:1-2页码:48-55 |
| 关键词 | High-performance Liquid Chromatography Gold Nanoparticle-initiated Chemiluminescence Monoamine Neurotransmitters Mouse Brain Microdialysates |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2009.04.050 |
| 英文摘要 | Our previous work showed that gold nanoparticles could trigger chemiluminescence (CL) between luminol and AgNO(3). In the present work, the effect of some biologically important reductive compounds, including monoamine neurotransmitters and their metabolites, reductive amino acids, ascorbic acid, uric acid, and glutathione, on the novel CL reaction were investigated for analytical purpose. It was found that all of them could inhibit the CL from the luminol-AgNO(3)-Au colloid system. Among them, monoamine neurotransmitters and their metabolites exhibited strong inhibition effect. Taking dopamine as a model compound, the CL mechanism was studied by measuring absorption spectra during the CL reaction and the reaction kinetics via stopped-flow technique. The CL inhibition mechanism is proposed to be due to that these tested compounds competed with luminol for AgNO(3) to inhibit the formation of luminol radicals and to accelerate deposition of Ag atoms on surface of gold nanoparticles, leading to a decrease in CL intensity. Based on the inhibited CL, a novel method for simultaneous determination of monoamine neurotransmitters and their metabolites was developed by coupling high-performance liquid chromatography with this CL reaction. The new method was successfully applied to determine the compounds in a mouse brain microdialysate. Compared with the reported HPLC-CL methods, the proposed method is simple, fast, and could determine more analytes. Moreover, the limits of linear ranges for NE, E, and DA using the proposed method were one order of magnitude lower than the luminol system without gold nanoparticles. (C) 2009 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000266952100008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68279]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Cui, Hua |
| 作者单位 | 1.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Na,Guo, Jizhao,Liu, Bo,et al. Determination of monoamine neurotransmitters and their metabolites in a mouse brain microdialysate by coupling high-performance liquid chromatography with gold nanoparticle-initiated chemiluminescence[J]. ANALYTICA CHIMICA ACTA,2009,645(1-2):48-55. |
| APA | Li, Na.,Guo, Jizhao.,Liu, Bo.,Yu, Yuqi.,Cui, Hua.,...&Lin, Yuqing.(2009).Determination of monoamine neurotransmitters and their metabolites in a mouse brain microdialysate by coupling high-performance liquid chromatography with gold nanoparticle-initiated chemiluminescence.ANALYTICA CHIMICA ACTA,645(1-2),48-55. |
| MLA | Li, Na,et al."Determination of monoamine neurotransmitters and their metabolites in a mouse brain microdialysate by coupling high-performance liquid chromatography with gold nanoparticle-initiated chemiluminescence".ANALYTICA CHIMICA ACTA 645.1-2(2009):48-55. |
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来源：化学研究所
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