Fluorescence-Amplifying Assay for Irradiated DNA Lesions Using Water-Soluble Conjugated Polymers
文献类型：期刊论文
| 作者 | Feng, Fude; Duan, Xinrui; Wang, Shu |
| 刊名 | MACROMOLECULAR RAPID COMMUNICATIONS
 |
| 出版日期 | 2009-02-02 |
| 卷号 | 30期号:3页码:147-151 |
| 关键词 | Conjugated Polymers Dna Lesions Fluorescence |
| ISSN号 | 1022-1336 |
| DOI | 10.1002/marc.200800543 |
| 英文摘要 | A new platform has been developed for DNA lesion detection using a cationic conjugated polymer (CCP). DNA that contains two adjacent thymine bases is irradiated with ultraviolet light to allow for the formation of cyclobutane pyrimidine dimers and pyrimidine-pyrimidone dimers. The DNA lesions block the primer extension, and the base labeled with fluorescein cannot be incorporated into the DNA strand. Addition of the CCP leads to inefficient fluorescence resonance energy transfer (FRET) from CCP to fluorescein. For the case without DNA lesions, successful primer extension allows for efficient FRET between them. In view of the FRET signal changes, the DNA lesions can be detected. This new protocol offers a convenient detection for DNA lesions in aqueous solution without any isolation and washing steps. |
| 语种 | 英语 |
| WOS记录号 | WOS:000263452400001 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68365]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Shu |
| 作者单位 | Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Fude,Duan, Xinrui,Wang, Shu. Fluorescence-Amplifying Assay for Irradiated DNA Lesions Using Water-Soluble Conjugated Polymers[J]. MACROMOLECULAR RAPID COMMUNICATIONS,2009,30(3):147-151. |
| APA | Feng, Fude,Duan, Xinrui,&Wang, Shu.(2009).Fluorescence-Amplifying Assay for Irradiated DNA Lesions Using Water-Soluble Conjugated Polymers.MACROMOLECULAR RAPID COMMUNICATIONS,30(3),147-151. |
| MLA | Feng, Fude,et al."Fluorescence-Amplifying Assay for Irradiated DNA Lesions Using Water-Soluble Conjugated Polymers".MACROMOLECULAR RAPID COMMUNICATIONS 30.3(2009):147-151. |
入库方式： OAI收割
来源：化学研究所
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