[Ru(bpy)(2)dppz](2+) Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor
文献类型：期刊论文
| 作者 | Hu, Lianzhe1,2; Bian, Zheng3; Li, Haijuan1,2; Han, Shuang1,2; Yuan, Yali1,2; Gao, Lianxun3; Xu, Guobao1 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 81期号:23页码:9807-9811 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac901925x |
| 英文摘要 | [Ru(bpy)(2)dppz](2+) electrochemilumineseence (ECL) was studied, and it was used to investigate DNA interaction and develop a label-free ATP aptasensor for the first time. ECL of [Ru(bpy)(2)dppz](2+) is negligible in aqueous solution, and increases similar to 1000 times when [Ru(bpy)(2)dppz](2+) intercalates into the nucleic acid structure. The ECL switch behavior of [Ru(bpy)(2)dppz](2+) is ascribed to the intercalation that shields the phenazine nitrogens from the solvent and results in a luminescent excited state. The ECL switch by DNA was applied to investigate the interaction of [Ru(bpy)(2)dppz](2+) with herring sperm DNA. The calculated equilibrium constant (K) is 1.35 x 10(6) M-1, and the calculated binding-site size (s) is 0.88 base pair, which is consistent with the reported values. Moreover, ATP can dramatically affect ECL of the [Ru(bpy)(2)dppz](2+)/ATP aptamer complex. As a result, a label-free, sensitive, and selective [Ru(bpy)(2)dppz](2+) ECL method for ATP detection was developed. The detection limit is 100 nM for ATP (with a signal-to-noise ratio, S/N, of 3) with a linear range of 0-1 mu M. ne result demonstrates that [Ru(bpy)(2)dppz](2+) ECL holds great promise in aptasensors. |
| 语种 | 英语 |
| WOS记录号 | WOS:000272084700043 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68455]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Xu, Guobao |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100864, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Jilin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Lianzhe,Bian, Zheng,Li, Haijuan,et al. [Ru(bpy)(2)dppz](2+) Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor[J]. ANALYTICAL CHEMISTRY,2009,81(23):9807-9811. |
| APA | Hu, Lianzhe.,Bian, Zheng.,Li, Haijuan.,Han, Shuang.,Yuan, Yali.,...&Xu, Guobao.(2009).[Ru(bpy)(2)dppz](2+) Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor.ANALYTICAL CHEMISTRY,81(23),9807-9811. |
| MLA | Hu, Lianzhe,et al."[Ru(bpy)(2)dppz](2+) Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor".ANALYTICAL CHEMISTRY 81.23(2009):9807-9811. |
入库方式： OAI收割
来源：化学研究所
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