中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Effect of refluxed silver nanoparticles on inhibition and enhancement of enzymatic activity of glucose oxidase

文献类型:期刊论文

作者Ma, Shiyu1,2; Mu, Jin1; Qu, Yi2; Jiang, Long3
刊名COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
出版日期2009-08-05
卷号345期号:1-3页码:101-105
关键词Glucose Oxidase Inhibitory Effect Enhancement Effect Silver Nanoparticles Reflux Interaction
ISSN号0927-7757
DOI10.1016/j.colsurfa.2009.04.038
英文摘要Glucose oxidase was immobilized on the surface of silver nanoparticles. The enzymatic activity of the glucose oxidase was determined by the refluxing time of silver nanoparticles. In a short refluxing time, the silver nanoparticles behaved to inhibit the enzymatic activity of the glucose oxidase. However, the inhibitory effect of silver nanoparticles reduced along with the refluxing time. And an enhancement effect appeared as the silver nanoparticles were refluxed in a certain period of time. When the refluxing time of silver nanoparticles was 3.0 h, the enzymatic activity of the immobilized glucose oxidase reached the maximum value, which was almost 4-fold as compared with that of the native glucose oxidase. Fluorescence and circular dichroism spectra indicated that the glucose oxidase on the surface of silver nanoparticles changed its conformation, and the conformation change depended on the refluxing time of silver nanoparticles, which was probably due to different interactions between glucose oxidase and silver nanoparticles. (C) 2009 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000268059800015
出版者ELSEVIER SCIENCE BV
源URL[http://ir.iccas.ac.cn/handle/121111/68487]  
专题中国科学院化学研究所
通讯作者Mu, Jin
作者单位1.E China Univ Sci & Technol, Dept Chem, Key Lab Adv Mat, Shanghai 200237, Peoples R China
2.E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China
3.Chinese Acad Sci, Inst Chem, Key Lab Colloid & Interface Sci, Beijing 100080, Peoples R China
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GB/T 7714
Ma, Shiyu,Mu, Jin,Qu, Yi,et al. Effect of refluxed silver nanoparticles on inhibition and enhancement of enzymatic activity of glucose oxidase[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2009,345(1-3):101-105.
APA Ma, Shiyu,Mu, Jin,Qu, Yi,&Jiang, Long.(2009).Effect of refluxed silver nanoparticles on inhibition and enhancement of enzymatic activity of glucose oxidase.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,345(1-3),101-105.
MLA Ma, Shiyu,et al."Effect of refluxed silver nanoparticles on inhibition and enhancement of enzymatic activity of glucose oxidase".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 345.1-3(2009):101-105.

入库方式: OAI收割

来源:化学研究所

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