Molecular mechanisms for interaction of glycine betaine with supra-molecular phycobiliprotein complexes
文献类型：期刊论文
| 作者 | Xu XiuLing; Li Heng; Xie Jie; Zhao JingQuan |
| 刊名 | SCIENCE IN CHINA SERIES B-CHEMISTRY
 |
| 出版日期 | 2009-11-01 |
| 卷号 | 52期号:11页码:1865-1870 |
| 关键词 | Glycine Betaine Phycobiliproteins Phycobilisome Supra-molecular Complex Molecular Interaction Mechanism |
| ISSN号 | 1006-9291 |
| DOI | 10.1007/s11426-009-0254-1 |
| 英文摘要 | Glycine betaine (GB) is a biologically important small molecule protecting cells, proteins and enzymes in vivo and in vitro under environmental stresses. Recently, it was found that GB could also relax the structure and inactivate the function of phycobiliproteins and phycobilisome (PBS), a kind of supramolecular complexes, in cyanobacterial cells. The molecular mechanisms for the opposite phenomena are quite ambiguous. Taking PBS and a trimeric or monomeric C-phycocyanin (C-PC) as models, the molecular mechanism for the interaction of GB with supra-molecular complexes or nuclear proteins was investigated. The energetic decoupling of PBS components induced by GB suggests that the PBS core-membrane linking polypeptide was the most sensitive site while the rod-core linker was the next. Biochemistry analysis proves that PBS structure was loosened but not dissociated into the components. On the basis of the results and structure knowledge, it was proposed that GB screened the electrostatic attraction of the opposite charges on a linker and a protein leading to a much looser structure. It was observed that GB induced a spectral blue shift for trimeric C-PC but a red shift for a monomeric C-PC (a nuclear protein), which were ascribed to GB's screening of the electrostatic attraction of a linker to a protein and strengthening of the hydrophobic interaction between C-PC monomers. The trimers and monomers' forming of the same products under high concentration of GB was ascribed to a compromise of the opposite interaction forces. |
| 语种 | 英语 |
| WOS记录号 | WOS:000272032700018 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68495]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao JingQuan |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Photochem Lab, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu XiuLing,Li Heng,Xie Jie,et al. Molecular mechanisms for interaction of glycine betaine with supra-molecular phycobiliprotein complexes[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(11):1865-1870. |
| APA | Xu XiuLing,Li Heng,Xie Jie,&Zhao JingQuan.(2009).Molecular mechanisms for interaction of glycine betaine with supra-molecular phycobiliprotein complexes.SCIENCE IN CHINA SERIES B-CHEMISTRY,52(11),1865-1870. |
| MLA | Xu XiuLing,et al."Molecular mechanisms for interaction of glycine betaine with supra-molecular phycobiliprotein complexes".SCIENCE IN CHINA SERIES B-CHEMISTRY 52.11(2009):1865-1870. |
入库方式： OAI收割
来源：化学研究所
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