Imaging Biocatalytic Activity of Enzyme-Polymer Spots by Means of Combined Scanning Electrochemical Microscopy/Electrogenerated Chemiluminescence
文献类型：期刊论文
| 作者 | Lei, Rong1,2; Stratmann, Lutz2; Schaefer, Dominik2; Erichsen, Thomas2; Neugebauer, Sebastian2; Li, Na1; Schuhmann, Wolfgang2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2009-06-15 |
| 卷号 | 81期号:12页码:5070-5074 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac900192n |
| 英文摘要 | The purpose of this study was to develop a scanning electrochemical microscopy (SECM) and scanning electrogenerated chemiluminescence (SECL) setup to visualize the localized enzymatic activity using glucose oxidase as a model. Combination of SECM and electrogenerated chemiluminescence (ECL) was made possible by integrating a photomultiplier tube (PMT) within a SECM setup which is mounted on top of an inverted microscope. An enzyme-polymer spot formed on a glass slide and placed on top of the entrance window of the PMT was used as a model sample to evaluate the potential of the combined SECM/ECL setup. Hydrogen peroxide, which was locally generated by the glucose oxidase (GOx)-catalyzed reaction, reacted with oxidized luminol which was simultaneously electrochemically generated at the positioned SECM electrode tip. By using the phase-sensitive lock-in amplifier, the potential applied to the SECM tip was sinusoidally swept to invoke an associated oscillation of the ECL. Thus, sensitivity of SECL could be substantially enhanced. Images of the local immobilized enzyme activity obtained both by ECL and generator/collector (GC) mode of SECM were compared to elucidate the pathway in which the SECM and SECL signals are generated. |
| 语种 | 英语 |
| WOS记录号 | WOS:000266969700051 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68579]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Na |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem, BNLMS,Minist Educ,Key Lab Bioorgan Chem Mol Engn, Beijing 100871, Peoples R China 2.Ruhr Univ Bochum, D-44780 Bochum, Germany |
| 推荐引用方式 GB/T 7714 | Lei, Rong,Stratmann, Lutz,Schaefer, Dominik,et al. Imaging Biocatalytic Activity of Enzyme-Polymer Spots by Means of Combined Scanning Electrochemical Microscopy/Electrogenerated Chemiluminescence[J]. ANALYTICAL CHEMISTRY,2009,81(12):5070-5074. |
| APA | Lei, Rong.,Stratmann, Lutz.,Schaefer, Dominik.,Erichsen, Thomas.,Neugebauer, Sebastian.,...&Schuhmann, Wolfgang.(2009).Imaging Biocatalytic Activity of Enzyme-Polymer Spots by Means of Combined Scanning Electrochemical Microscopy/Electrogenerated Chemiluminescence.ANALYTICAL CHEMISTRY,81(12),5070-5074. |
| MLA | Lei, Rong,et al."Imaging Biocatalytic Activity of Enzyme-Polymer Spots by Means of Combined Scanning Electrochemical Microscopy/Electrogenerated Chemiluminescence".ANALYTICAL CHEMISTRY 81.12(2009):5070-5074. |
入库方式： OAI收割
来源：化学研究所
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