The bioactivity of rhBMP-2 immobilized poly(lactide-co-glycolide) scaffolds
文献类型：期刊论文
| 作者 | Shen, Hong; Hu, Xixue; Yang, Fei; Bei, Jianzhong; Wang, Shenguo |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 30期号:18页码:3150-3157 |
| 关键词 | Rhbmp-2 Oxygen Plasma Treatment Anchorage Immobilization Plga Scaffold Tissue Engineering |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2009.02.004 |
| 英文摘要 | In this study, immobilization of rhBMP-2 on polylactone-type polymer scaffolds via plasma treatment was investigated. To introduce proper functional groups on the surface of poly(lactide-co-glycolide) (PLGA) matrix, PLGA films were treated under different atmospheres, such as oxygen, ammonia and carbon dioxide, respectively, and then incubated in rhBMP-2 solution of de-ionized water. The effect of various plasma-treated PLGA films on binding rhBMP-2 was investigated and compared. It was found that PLGA binding ability to rhBMP-2 was enhanced by carbon dioxide and oxygen plasma treatment, and the binding ability of the oxygen plasma-treated PLGA (OT-PLGA) to rhBMP-2 was the strongest after oxygen plasma treating for 10 min under a power of 50 W. The changes of surface chemistry and surface topography of PLGA matrix induced by oxygen plasma treatment played main roles in improving the PLGA binding ability to rhBMP-2. The stability of rhBMP-2 bound on OT-PLGA film was determined under a dynamic condition by a Parallel Plate Flow Chamber. The result showed that the rhBMP-2 had been immobilized on the OT-PLGA film. Mouse OCT-1 osteoblast-like cell as a model cell was cultured on the rhBMP-2 bound OT-PLGA (OT-PLGA/BMP) in vitro, which showed that the bound rhBMP-2 via oxygen plasma treatment was bioactive. Depending on hydrophilicity and rich polar O-containing groups of the OT-PLGA scaffold, different amount of rhBMP-2 could be evenly immobilized on the surface of the OT-PLGA scaffold. The immobilized rhBMP-2 had stimulated differentiation of OCT-1 cell and accelerated process of mineralization of OCT-1 cell in the scaffold. It revealed the rhBMP-2 immobilized PLGA scaffold had good cell affinity. (C) 2009 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000266148200004 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68623]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Shenguo |
| 作者单位 | Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, BNLMS, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Hong,Hu, Xixue,Yang, Fei,et al. The bioactivity of rhBMP-2 immobilized poly(lactide-co-glycolide) scaffolds[J]. BIOMATERIALS,2009,30(18):3150-3157. |
| APA | Shen, Hong,Hu, Xixue,Yang, Fei,Bei, Jianzhong,&Wang, Shenguo.(2009).The bioactivity of rhBMP-2 immobilized poly(lactide-co-glycolide) scaffolds.BIOMATERIALS,30(18),3150-3157. |
| MLA | Shen, Hong,et al."The bioactivity of rhBMP-2 immobilized poly(lactide-co-glycolide) scaffolds".BIOMATERIALS 30.18(2009):3150-3157. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。