Characterizing DNA Condensation and Conformational Changes in Organic Solvents
文献类型：期刊论文
| 作者 | Ke, Fuyou1,2; Luu, Yen Kim3; Hadjiargyrou, Michael3; Liang, Dehai1,2 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2010-10-11 |
| 卷号 | 5期号:10 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0013308 |
| 英文摘要 | Organic solvents offer a new approach to formulate DNA into novel structures suitable for gene delivery. In this study, we examined the in situ behavior of DNA in N, N-dimethylformamide (DMF) at low concentration via laser light scattering (LLS), TEM, UV absorbance and Zeta potential analysis. Results revealed that, in DMF, a 21bp oligonucleotide remained intact, while calf thymus DNA and supercoiled plasmid DNA were condensed and denatured. During condensation and denaturation, the size was decreased by a factor of 8-10, with calf thymus DNA forming spherical globules while plasmid DNA exhibited a toroid-like conformation. In the condensed state, DNA molecules were still able to release the counterions to be negatively charged, indicating that the condensation was mainly driven by the excluded volume interactions. The condensation induced by DMF was reversible for plasmid DNA but not for calf thymus DNA. When plasmid DNA was removed from DMF and resuspended in an aqueous solution, the DNA was quickly regained a double stranded configuration. These findings provide further insight into the behavior and condensation mechanism of DNA in an organic solvent and may aid in developing more efficient non-viral gene delivery systems. |
| 语种 | 英语 |
| WOS记录号 | WOS:000282748100030 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68709]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ke, Fuyou |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Polymer Chem & Phys, Beijing 100871, Peoples R China 3.SUNY Stony Brook, Dept Biomed Engn, Stony Brook, NY 11794 USA |
| 推荐引用方式 GB/T 7714 | Ke, Fuyou,Luu, Yen Kim,Hadjiargyrou, Michael,et al. Characterizing DNA Condensation and Conformational Changes in Organic Solvents[J]. PLOS ONE,2010,5(10). |
| APA | Ke, Fuyou,Luu, Yen Kim,Hadjiargyrou, Michael,&Liang, Dehai.(2010).Characterizing DNA Condensation and Conformational Changes in Organic Solvents.PLOS ONE,5(10). |
| MLA | Ke, Fuyou,et al."Characterizing DNA Condensation and Conformational Changes in Organic Solvents".PLOS ONE 5.10(2010). |
入库方式： OAI收割
来源：化学研究所
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