Polymer mixtures with enhanced compatibility and extremely low viscosity used as DNA separation media
文献类型：期刊论文
| 作者 | Ke, Fuyou1; Mo, Xiulei1; Yang, Runmiao2; Wang, Yanmei2; Liang, Dehai1 |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 31期号:3页码:520-527 |
| 关键词 | Dna Separation Electrophoresis Laser Light Scattering Polymer Mixtures Viscometry |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.200900440 |
| 英文摘要 | Poor compatibility was the major drawback of polymer mixtures when used as DNA separation media. Using poly(ethylene oxide)-b-poly(N, N-dimethylacrylamide) (PEO(44)-b-PDMA(88)) and PEO (M(w): 1.3 MDa) as an example, we demonstrated the concept that the compatibility was significantly improved when mixing a homopolymer with its copolymer. Laser light scattering indicated that the major interaction between PEO and PEO-b-PDMA in dilute solution was the weak hydrodynamic interaction, which showed almost no effect on the viscosity and the static scattering pattern. In semidilute or concentrated solution, viscosity measurement also suggested good compatibility between the two components. When tested as DNA separation medium by CE, the viscosity of the mixture was extremely low, only 5 cP for 5.0 m/v% PEO-b-PDMA+0.1 m/v% PEO at 25 degrees C. The performance on DNA separation could be tuned by varying the concentration of each component as well as the component ratio. Good separation on both dsDNA and ssDNA was achieved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000274603200011 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68881]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liang, Dehai |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Polymer Chem & Phys,Beijing Natl Lab Mol, Beijing 100871, Peoples R China 2.Univ Sci & Technol China, Dept Polymer Sci & Engn, Hefei 230026, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ke, Fuyou,Mo, Xiulei,Yang, Runmiao,et al. Polymer mixtures with enhanced compatibility and extremely low viscosity used as DNA separation media[J]. ELECTROPHORESIS,2010,31(3):520-527. |
| APA | Ke, Fuyou,Mo, Xiulei,Yang, Runmiao,Wang, Yanmei,&Liang, Dehai.(2010).Polymer mixtures with enhanced compatibility and extremely low viscosity used as DNA separation media.ELECTROPHORESIS,31(3),520-527. |
| MLA | Ke, Fuyou,et al."Polymer mixtures with enhanced compatibility and extremely low viscosity used as DNA separation media".ELECTROPHORESIS 31.3(2010):520-527. |
入库方式： OAI收割
来源：化学研究所
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