Noncovalent Attachment of NAD(+) Cofactor onto Carbon Nanotubes for Preparation of Integrated Dehydrogenase-Based Electrochemical Biosensors
文献类型：期刊论文
| 作者 | Zhou, Haojie1; Zhang, Zipin1; Yu, Ping1; Su, Lei1; Ohsaka, Takeo2; Mao, Lanqun1 |
| 刊名 | LANGMUIR
 |
| 出版日期 | 2010-04-20 |
| 卷号 | 26期号:8页码:6028-6032 |
| ISSN号 | 0743-7463 |
| DOI | 10.1021/la903799n |
| 英文摘要 | This study describes a facile approach to the preparation of integrated dehydrogenase-based electrochemical biosensors through noncovalent attachment (Tan oxidized form of beta-nicotinamide adenine dinucleotide (NAD(+)) onto carbon nanotubes with the interaction between the adenine subunit in NAD(+) molecules and multiwalled carbon nanotubes (MWCNTs). X-ray photoelectron spectroscopic and cyclic voltammetric results suggest that NAD(+) is noncovalently attached onto MWCNTs to form an NAD(+)/MWCNT composite that acts as the electronic transducer for the integrated dehydrogenase-based electrochemical biosensors. With glucose dehydrogenase (GDH) as a model dehydrogenase-based recognition unit, electrochemical studies reveal that glucose is readily oxidized at the GDH/NAD(+)/MWCNT-modified electrode without addition of NAD(+) in the phosphate buffer. The potential for the oxidation of glucose at the GDH/NAD(+)/MWCNT-modified electrode remains very close to that for NADH oxidation at the MWCNT-modified electrode, but it is more negative than those for the oxidation of glucose at the MWCNT-modified electrode and for NADH oxidation at a bare glassy carbon electrode. These results demonstrate that NAD(+) molecules stably attached onto MWCNTs efficiently act as the cofactor for the dehydrogenases. MWCNTs employed here not only serve as the electronic transducer and the support to confine NAD(+) cofactor onto the electrode surface, hut also act as the electrocatalyst For NADH oxidation in the dehydrogenase-based electrochemical biosensors. At the GDH/NAD(+)/MWCNT-based glucose biosensor, the current is linear with the concentration of glucose being within a concentration ranee from 10 to 300 mu M with a limit of detection down to 4.81 mu M (S/N = 3). This study offers a facile and versatile approach to the development of integrated dehydrogenase-based electrochemical devices, such as electrochemical biosensors and biofuel cells. |
| 语种 | 英语 |
| WOS记录号 | WOS:000276562300098 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68987]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Mao, Lanqun |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 2.Tokyo Inst Technol, Dept Elect Chem, Interdisciplinary Grad Sch Sci & Engn, Midori Ku, Yokohama, Kanagawa 2268502, Japan |
| 推荐引用方式 GB/T 7714 | Zhou, Haojie,Zhang, Zipin,Yu, Ping,et al. Noncovalent Attachment of NAD(+) Cofactor onto Carbon Nanotubes for Preparation of Integrated Dehydrogenase-Based Electrochemical Biosensors[J]. LANGMUIR,2010,26(8):6028-6032. |
| APA | Zhou, Haojie,Zhang, Zipin,Yu, Ping,Su, Lei,Ohsaka, Takeo,&Mao, Lanqun.(2010).Noncovalent Attachment of NAD(+) Cofactor onto Carbon Nanotubes for Preparation of Integrated Dehydrogenase-Based Electrochemical Biosensors.LANGMUIR,26(8),6028-6032. |
| MLA | Zhou, Haojie,et al."Noncovalent Attachment of NAD(+) Cofactor onto Carbon Nanotubes for Preparation of Integrated Dehydrogenase-Based Electrochemical Biosensors".LANGMUIR 26.8(2010):6028-6032. |
入库方式： OAI收割
来源：化学研究所
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