Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles
文献类型：期刊论文
| 作者 | Wang, Xinyi2; Xu, Yao1; Xu, Xiao1; Hu, Ke1; Xiang, Minghui1; Li, Limei2; Liu, Feng1; Li, Na1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2010-07-15 |
| 卷号 | 82期号:2页码:693-697 |
| 关键词 | Lysozyme Plasmon Resonance Light-scattering Gold Nanoparticles Urinary Sample |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2010.05.034 |
| 英文摘要 | The purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4 nM citrate-capped AuNPs (13 nm in diameter), the linear range of the calibration curve was 15-50 nM with a detection limit of 13.1 nM for lysozyme. Six nanomolar lysozyme can produce an observable PRLS enhancement. Most potential interfering substances present in urine had a negligible effect on the determination. The interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at pH 4.8-4.9. The 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. This simple and sensitive method for lysozyme does not require sample clean-up and AuNPs modification, thus provided an alternative for urinary lysozyme determination. (C) 2010 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000280375700040 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/68999]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Na |
| 作者单位 | 1.Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Shenyang Agr Univ, Coll Sci, Shenyang 110161, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xinyi,Xu, Yao,Xu, Xiao,et al. Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles[J]. TALANTA,2010,82(2):693-697. |
| APA | Wang, Xinyi.,Xu, Yao.,Xu, Xiao.,Hu, Ke.,Xiang, Minghui.,...&Li, Na.(2010).Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles.TALANTA,82(2),693-697. |
| MLA | Wang, Xinyi,et al."Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles".TALANTA 82.2(2010):693-697. |
入库方式： OAI收割
来源：化学研究所
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