Adsorptive Immobilization of Four Proteases on Different Molecular Sieves
文献类型：期刊论文
| 作者 | Liu Ping; Xing Guo-Wen; Li Xuan-Wen; Ye Yun-Hua |
| 刊名 | ACTA PHYSICO-CHIMICA SINICA
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 26期号:4页码:1113-1118 |
| ISSN号 | 1000-6818 |
| 关键词 | Adsorption Immobilization Protease Zeolite Molecular Sieve Peptide Synthesis |
| DOI | 10.3866/PKU.WHXB20100448 |
| 英文摘要 | The immobilization of four different proteases on a series of molecular sieves, including microporous HY, NaY, NH4Y, MCM-22, H beta zeolites, modified Y zeolites HDAY, HNH(4)DAY, and mesoporous MCM-41, by adsorption was systematically investigated The four proteases were alpha-chymotrypsin, papain, subtilisin, and thermoase (or its pure form thermolysin) The results showed that the enzyme loading amount and the activity of immobilized enzyme were significantly affected not only by the structures and textures of molecular sieves and the enzyme properties. but also by the adsorptive conditions such as buffer pH values and enzyme concentration In most cases, the amount of protease loading on molecular sieves was relatively higher at pH 6 0, but declined with further increasing of pH values The nature of the interaction between protease and molecular sieves is discussed As for alpha-chymotrypsin, its loading amount on H beta zeolites was found to be the highest, whereas the activity of a-chymotrypsin immobilized on MCM-22 was the highest, which is probably due to different adsorption states. |
| 语种 | 英语 |
| 出版者 | PEKING UNIV PRESS |
| WOS记录号 | WOS:000276958400041 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/69063]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ye Yun-Hua |
| 作者单位 | Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Ping,Xing Guo-Wen,Li Xuan-Wen,et al. Adsorptive Immobilization of Four Proteases on Different Molecular Sieves[J]. ACTA PHYSICO-CHIMICA SINICA,2010,26(4):1113-1118. |
| APA | Liu Ping,Xing Guo-Wen,Li Xuan-Wen,&Ye Yun-Hua.(2010).Adsorptive Immobilization of Four Proteases on Different Molecular Sieves.ACTA PHYSICO-CHIMICA SINICA,26(4),1113-1118. |
| MLA | Liu Ping,et al."Adsorptive Immobilization of Four Proteases on Different Molecular Sieves".ACTA PHYSICO-CHIMICA SINICA 26.4(2010):1113-1118. |
入库方式： OAI收割
来源：化学研究所
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