Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis
文献类型：期刊论文
| 作者 | Liang, Yuan; Zhou, Shuang; Hu, Liming; Li, Lin; Zhao, Meiping; Liu, Huwei |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2010-01-15 |
| 卷号 | 878期号:2页码:278-282 |
| 关键词 | Immunoaffinity Monolith Class-specific Pyrethroid On-line Clean-up |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2009.06.007 |
| 英文摘要 | A class-specific monolithic immunoaffinity column was developed for on-line clean-up of pyrethroid insecticides in complex samples Deltamethrin was oxidized with ozone to generate the hapten of (RS)-acyano-alpha-phenoxybenzyl (RS)-cis,traps-2,2-dimethyl-3-formyl-cyclopropane carboxylate Class-specific antibodies against pyrethroids were produced using the conjugate of above hapten with bovine serum albumin as the immunogen. Poly(ethylene dimethacrylate-glycidyl methacrylate) monolith was synthesized in a 50 mm, 4.6 mm id stainless steel cartridge with two auxiliary pipette tips. The polymerization method was proved to be economic and reproducible. Antibodies against pyrethrotds were covalently immobilized onto the monolithic support via Schiff base reaction With a column-switching valve system, the immunoaffinity monolith (IAM) could be readily adapted to the reversed-phase high-performance liquid chromatography (HPLC) system. Under the optimum loading, washing and eluting conditions, the IAM specifically retained deltamethrin, flumethrin. flucythrinate and cis/traps permethrin, which were further baseline separated by C18 column using acetonitrile-water (83:17, V/V) as the mobile phase at a flow rate of 1.0 mL(min. The established system provides a highly efficient approach for high-throughput on-line clean-up of pyrethroid in various samples. (C) 2009 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000274220900022 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/69273]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Meiping |
| 作者单位 | Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, BNLMS,Minist Educ, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Yuan,Zhou, Shuang,Hu, Liming,et al. Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(2):278-282. |
| APA | Liang, Yuan,Zhou, Shuang,Hu, Liming,Li, Lin,Zhao, Meiping,&Liu, Huwei.(2010).Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,878(2),278-282. |
| MLA | Liang, Yuan,et al."Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 878.2(2010):278-282. |
入库方式： OAI收割
来源：化学研究所
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