Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex
文献类型：期刊论文
| 作者 | Pu, Fang1; Hu, Dan1; Ren, Jinsong1; Wang, Shu2; Qu, Xiaogang1 |
| 刊名 | LANGMUIR
 |
| 出版日期 | 2010-03-16 |
| 卷号 | 26期号:6页码:4540-4545 |
| ISSN号 | 0743-7463 |
| DOI | 10.1021/la904173j |
| 英文摘要 | By taking advantage of the large signal amplification through efficient fluorescence resonance energy transfer (FRET) from the conjugated polymer to the intercalating dye mediated by DNA, a new strategy for nuclease assay has been developed using conjugated polymer and DNA/intercalating dye complex. The discrimination of DNA before or after digestion by nuclease denotes the universal application of the approach, in which either dsDNA or ssDNA substrates could be used for detecting nuclease activity. This method can be extended to most nucleases by simply changing the Substrate DNA. The present method is label-free, rapid,and highly sensitive, with a detection limit Much better or at least comparable to previous reports. fit addition, this assay is easy to implement for Visual detection with the assistance of a UV transilluminator. We reason that a similar strategy can be adopted for the detection of other analytes. |
| 语种 | 英语 |
| WOS记录号 | WOS:000275226700111 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/69345]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ren, Jinsong |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Rare Earth Resources Utilizat, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Key Lab Organ Solids, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pu, Fang,Hu, Dan,Ren, Jinsong,et al. Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex[J]. LANGMUIR,2010,26(6):4540-4545. |
| APA | Pu, Fang,Hu, Dan,Ren, Jinsong,Wang, Shu,&Qu, Xiaogang.(2010).Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex.LANGMUIR,26(6),4540-4545. |
| MLA | Pu, Fang,et al."Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex".LANGMUIR 26.6(2010):4540-4545. |
入库方式： OAI收割
来源：化学研究所
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