Transmembrane Delivery of Aggregated [Gd@C-82(OH)(22)](n) Nanoparticles
文献类型：期刊论文
| 作者 | Zhang, Mingyi1,2; Xing, Gengmei1,2; Yuan, Hui1,2; Chang, Xueling1,2; Jing, Long1,2; Zhao, Yuliang1,2; Zhu, Chuanfeng3; Fang, Xiaohong3 |
| 刊名 | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
 |
| 出版日期 | 2010-12-01 |
| 卷号 | 10期号:12页码:8556-8561 |
| 关键词 | Transmembrane Delivery Gd@c-82(Oh)(22) Erythrocyte |
| ISSN号 | 1533-4880 |
| DOI | 10.1166/jnn.2010.2490 |
| 英文摘要 | We investigated whether multi-hydroxyl metallofullerenes can penetrate into erythrocyte and whether this potential transmembrane delivery requires aggregated nanostructure of these particles. The metal atom encapsulated in metallofullerenes was used as a quantitative marker to investigate body distribution of aggregated nanoparticles in cytomembrane and cytoplasm. Image of atomic force microscopy (AFM) and assay of inductively coupled plasma-mass spectrometry (ICP-MS) suggested that aggregated 22 [Gd@C-82(OH)(22)](n) particles traversed through cytomembrane into belonged cytoplasm. Aggregated Gd nanostructure to small sphere with average diameter of, 22.4 +/- 0.5 nm. For pristine Gd@C-82 molecule, due to the electron donation from Gd atom, the distribution of electrons on the surface of carbon cage was localized. The electrophilic additive reaction of polyhydroxyl on the surface of Gd@C-82 was directly affected by local distribution of electrons. This resulted in local distribution of hydroxyls on the surface of Gd nanoparticles. Local distribution of hydroxyls brought about polar and nonpolar domains on particle surface, which induced Gd@C-82(OH)(22) to be amphiphilic molecule with due hydrophilic and hydrophobic properties. Amphiphilic properties of these molecules promoted their mutual aggregation in water. In the process of aggregation, amphiphilic properties of aggregated nanoparticles were well maintained, and besides, hydrophilic and hydrophobic domains were also regularly distributed on the surface of 22, [Gd@C-82(OH)(22)](n) particles. The amphiphilic nanoparticles attached externally to cytomembrane of, erythrocyte might be effectively driven by hydrophobic effect when they directly contacted cytomembrane of erythrocyte. The number of 22, [Gd@C-82(OH)(22)](n) nanoparticles attached to cytomembrane,n reached up to a certain crilical threshold, a significant curvature tension of membrane would occur. The shape of cell was accordingly changed. Increased membrane tension triggered the sudden opening of specific pores as a result of cytmennbrane response to nanoparitcle effect and the 22 [Gd@C-82(OH)(22)](n) nanoparticles gained entry to cell via these pores. This process was biologically independent of caveolar-mediated endocytosis or transportation pathway via ion channels. |
| 语种 | 英语 |
| WOS记录号 | WOS:000283621400093 |
| 出版者 | AMER SCIENTIFIC PUBLISHERS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/69371]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Xing, Gengmei |
| 作者单位 | 1.Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 2.Natl Ctr Nanosci & Technol China, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Mingyi,Xing, Gengmei,Yuan, Hui,et al. Transmembrane Delivery of Aggregated [Gd@C-82(OH)(22)](n) Nanoparticles[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2010,10(12):8556-8561. |
| APA | Zhang, Mingyi.,Xing, Gengmei.,Yuan, Hui.,Chang, Xueling.,Jing, Long.,...&Fang, Xiaohong.(2010).Transmembrane Delivery of Aggregated [Gd@C-82(OH)(22)](n) Nanoparticles.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,10(12),8556-8561. |
| MLA | Zhang, Mingyi,et al."Transmembrane Delivery of Aggregated [Gd@C-82(OH)(22)](n) Nanoparticles".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 10.12(2010):8556-8561. |
入库方式： OAI收割
来源：化学研究所
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