Recognition of Single- and Double-Stranded Oligonucleotides by Bovine Serum Albumin via Nonspecific Interactions
文献类型：期刊论文
| 作者 | Xia, Yuqiong; Chen, Erqiang; Liang, Dehai1 |
| 刊名 | BIOMACROMOLECULES
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 11期号:11页码:3158-3166 |
| ISSN号 | 1525-7797 |
| DOI | 10.1021/bm100969z |
| 英文摘要 | The complexes formed by bovine serum albumin (BSA) with single-stranded oligonucleotide (ss-oligo) or double-stranded oligonucleotide (ds-oligo) were investigated by laser light scattering, zeta potential analysis, and atomic force microscopy. It was found that BSA was able to recognize ss-oligo and ds-oligo upon forming complexes in HCOOH-HCOONa buffer at pH 3.0. When oligonucleotide was added dropwisc to BSA. BSA formed a complex with ss-oligo but not with ds-oligo in the studied charge ratio. When BSA was added to oligonucleotides. BSA formed complexes with both ss-oligo and ds-oligo but via different paths: the BSA/ds-oligo underwent two processes, heavy precipitation followed by reentry, with increasing BSA/oligo charge ratio, whereas BSA/ss-oligo underwent only aggregation process. but with a charge reversal occurred at BSA/oligo charge ratio about 0.1. Moreover, the complex formed by BSA and ds-oligo showed a kinetics much slower than that of BSA and ss-oligo. We attributed the big difference upon complexation to the physical nature of oligonucleotides as well as the conformational change of BSA under severe conditions. The differentiation of ss-oligo from ds-oligo by BSA via nonspecific interactions gained insight in the recognition of DNA or RNA by specific protein (enzyme) under physiological conditions. |
| 语种 | 英语 |
| WOS记录号 | WOS:000283810900043 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/69491]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liang, Dehai |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Polymer Chem & Phys, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Yuqiong,Chen, Erqiang,Liang, Dehai. Recognition of Single- and Double-Stranded Oligonucleotides by Bovine Serum Albumin via Nonspecific Interactions[J]. BIOMACROMOLECULES,2010,11(11):3158-3166. |
| APA | Xia, Yuqiong,Chen, Erqiang,&Liang, Dehai.(2010).Recognition of Single- and Double-Stranded Oligonucleotides by Bovine Serum Albumin via Nonspecific Interactions.BIOMACROMOLECULES,11(11),3158-3166. |
| MLA | Xia, Yuqiong,et al."Recognition of Single- and Double-Stranded Oligonucleotides by Bovine Serum Albumin via Nonspecific Interactions".BIOMACROMOLECULES 11.11(2010):3158-3166. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。