The role of calcium ions in the interactions of PrP106-126 amide with model membranes
文献类型：期刊论文
| 作者 | Zhong, Jian1,2; Yang, Chunhui1,2,3; Zheng, Wenfu1,2; Huang, Lixin1,2; Hong, Yuankai1,2; Wang, Lijun1,2; Sha, Yinlin1,2 |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2010-05-01 |
| 卷号 | 77期号:1页码:40-46 |
| 关键词 | Ca2++++ Prp106-126 Amide Poration Membrane Disintegration Atomic Force Microscopy |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2010.01.001 |
| 英文摘要 | In this work, we investigated the interactions of PrP106-126 amide with 1-palmitoyl-2-oleoyl-3-phosphocholine (POPC) and POPC/bovine brain sphingomyelin (BSM) membranes in the presence of calcium ions by in situ time-lapse atomic force microscopy (AFM) and circular dichroism (CD). The CD results show that Ca2+ has no obvious effects on the random coil conformation of PrP106-126 amide. The tapping mode AFM results demonstrate that electrostatic interaction decreases the measured heights of supported lipid bilayers (SLBs) in HBS-Ca2+ solution. Electrostatic interaction analysis also can be used to determine the applied force in liquid tapping mode AFM. The interactions of PrP106-126 amide with membranes by AFM demonstrate the following: (i)Ca2+ inhibits the interaction of PrP106-126 amide with POPC lipid and (ii) the co-interaction between Ca2+ and BSM increases the poration ability of PrP106-126 amide. These results imply that the main role of Ca2+ in the interactions of PrP106-126 amide with membranes is changing the surface properties of the membranes. (C) 2010 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000276272100006 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/69523]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhong, Jian |
| 作者单位 | 1.Peking Univ, Dept Biophys, Singlemol & Nanobiol Lab, Sch Basic Med Sci, Beijing 100191, Peoples R China 2.Peking Univ, Biomed X Ctr, Beijing 100191, Peoples R China 3.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, Jian,Yang, Chunhui,Zheng, Wenfu,et al. The role of calcium ions in the interactions of PrP106-126 amide with model membranes[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2010,77(1):40-46. |
| APA | Zhong, Jian.,Yang, Chunhui.,Zheng, Wenfu.,Huang, Lixin.,Hong, Yuankai.,...&Sha, Yinlin.(2010).The role of calcium ions in the interactions of PrP106-126 amide with model membranes.COLLOIDS AND SURFACES B-BIOINTERFACES,77(1),40-46. |
| MLA | Zhong, Jian,et al."The role of calcium ions in the interactions of PrP106-126 amide with model membranes".COLLOIDS AND SURFACES B-BIOINTERFACES 77.1(2010):40-46. |
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来源：化学研究所
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