Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis
文献类型：期刊论文
| 作者 | Xie Ming-Yi1; Guo Zhen-Peng1; Chen Yi1,2 |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2010-11-10 |
| 卷号 | 31期号:11页码:2162-2166 |
| 关键词 | Capillary Electrophoresis Gold Nanoparticle Bovine Serum Albumin Interaction Constant |
| ISSN号 | 0251-0790 |
| 英文摘要 | A capillary electrophoretic method was developed to study the interaction of gold nanoparticles (GNPs) with bovine serum albumin(BSA), capable of determining the binding constant and binding capacity. For demonstration citric acid-protected GNPs were tried, the measured bonding constant was 28.6 L/mu mol and the saturated binding capacity was 24 BSA molecules per GNP, corresponding to a surface concentration of 4.5 x 10(12) BSA/cm(2). The determination was shown to depend on the incubating pH value and the concentration of run buffer which should be optimized accordingly. It was found that the peaks of the citric acid-protected GNPs were commonly broad, overlaid with irreproducible bars, but could easily be improved by addition of a small amount of BSA. The established method is expected to be extendable to inspecting the interaction of GNPs with other biomolecules. |
| 语种 | 英语 |
| WOS记录号 | WOS:000285538100011 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/69649]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen Yi |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 2.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie Ming-Yi,Guo Zhen-Peng,Chen Yi. Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2010,31(11):2162-2166. |
| APA | Xie Ming-Yi,Guo Zhen-Peng,&Chen Yi.(2010).Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,31(11),2162-2166. |
| MLA | Xie Ming-Yi,et al."Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 31.11(2010):2162-2166. |
入库方式： OAI收割
来源：化学研究所
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