Monomeric type I and type III transforming growth factor-beta receptors and their dimerization revealed by single- molecule imaging
文献类型：期刊论文
| 作者 | Zhang, Wei1; Yuan, Jinghe1; Yang, Yong1; Xu, Li1; Wang, Qiang2; Zuo, Wei2; Fang, Xiaohong1; Chen, Ye-Guang2 |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 20期号:11页码:1216-1223 |
| 关键词 | Single-molecule Fluorescence Tgf-beta Signaling Type i Tgf-beta Receptor Type Iii Tgf-beta Receptor Subunit Stoichiometry |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2010.105 |
| 英文摘要 | Transforming growth factor-beta (TGF-beta) binds with two transmembrane serine/threonine kinase receptors, type II (T beta RII) and type I receptors (T beta RI), and one accessory receptor, type III receptor (T beta RIII), to transduce signals across cell membranes. Previous biochemical studies suggested that T beta RI and T beta RIII are preexisted homo-dimers. Using single-molecule microscopy to image green fluorescent protein-labeled membrane proteins, for the first time we have demonstrated that T beta RI and T beta RIII could exist as monomers at a low expression level. Upon TGF-beta 1 stimulation, T beta RI follows the general ligand-induced receptor dimerization model for activation, but this process is T beta RII-dependent. The monomeric status of the non-kinase receptor T beta RIII is unchanged in the presence of TGF-beta 1. With the increase of receptor expression, both T beta RI and T beta RIII can be assembled into dimers on cell surfaces. |
| 语种 | 英语 |
| WOS记录号 | WOS:000283713000007 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/69679]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, Xiaohong |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Mol Nanostruct & Nanotechnol, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Tsinghua Univ, Sch Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wei,Yuan, Jinghe,Yang, Yong,et al. Monomeric type I and type III transforming growth factor-beta receptors and their dimerization revealed by single- molecule imaging[J]. CELL RESEARCH,2010,20(11):1216-1223. |
| APA | Zhang, Wei.,Yuan, Jinghe.,Yang, Yong.,Xu, Li.,Wang, Qiang.,...&Chen, Ye-Guang.(2010).Monomeric type I and type III transforming growth factor-beta receptors and their dimerization revealed by single- molecule imaging.CELL RESEARCH,20(11),1216-1223. |
| MLA | Zhang, Wei,et al."Monomeric type I and type III transforming growth factor-beta receptors and their dimerization revealed by single- molecule imaging".CELL RESEARCH 20.11(2010):1216-1223. |
入库方式： OAI收割
来源：化学研究所
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