Polystyrene spheres coated with gold nanoparticles for detection of DNA
文献类型：期刊论文
| 作者 | Li, Shaohua; Xia, Yuetong; Zhang, Jianping; Han, Jianhua; Jiang, Long |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 31期号:18页码:3090-3096 |
| 关键词 | Dna Hybridization Dna Immobilization Polystyrene Spheres Coated With Gold Nanoparticles Quartz Crystal Microbalance |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.201000204 |
| 英文摘要 | A novel method has been developed here utilizing gold-modified polystyrene spheres (PS@Au) as carriers for the investigation of DNA hybridization. The immobilization ability of DNA probes on the PS@Au was investigated by a quartz crystal microbalance (QCM). More gold nanoparticles (AuNPs) were conjugated to the QCM surface in the form of PS@Au as compared with the AuNPs alone. The influence of PS, AuNPs population and size on the DNA probe fixation, and hybridization has been investigated. The results show that more AuNPs were achievable onto the QCM surface and could greatly increase the immobilization amount further hybridization amount of DNA. There was an optimal surface curvature for the probe DNA absorption. The curvature about 12 nm was more suited to the adsorption of probe DNA which could lead to the detection limit further down to 10(-12)M. These results demonstrate that the introduction of PS@Au, with polystyrene spheres as the medium between the QCM surface and the AuNPs, could achieve modified QCM surfaces and increase its detection ability for a better DNA assay and a novel platform for the fabrication of DNA sensors. |
| 语种 | 英语 |
| WOS记录号 | WOS:000283389900011 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/69961]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jiang, Long |
| 作者单位 | Chinese Acad Sci, Inst Chem, Key Lab Colloid & Interface Sci, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shaohua,Xia, Yuetong,Zhang, Jianping,et al. Polystyrene spheres coated with gold nanoparticles for detection of DNA[J]. ELECTROPHORESIS,2010,31(18):3090-3096. |
| APA | Li, Shaohua,Xia, Yuetong,Zhang, Jianping,Han, Jianhua,&Jiang, Long.(2010).Polystyrene spheres coated with gold nanoparticles for detection of DNA.ELECTROPHORESIS,31(18),3090-3096. |
| MLA | Li, Shaohua,et al."Polystyrene spheres coated with gold nanoparticles for detection of DNA".ELECTROPHORESIS 31.18(2010):3090-3096. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。