Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide
文献类型：期刊论文
| 作者 | Wang, Hu1; Zhong, Chun-Yan1; Wu, Jiang-Feng1; Huang, Yu-Bin2; Liu, Chang-Bai1 |
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2010-04-02 |
| 卷号 | 143期号:1页码:64-70 |
| 关键词 | Cell Penetrating Peptides (Cpps) Dimethylsulfoxide (Dmso) Tat Penetration |
| ISSN号 | 0168-3659 |
| DOI | 10.1016/j.jconrel.2009.12.003 |
| 英文摘要 | Cell penetrating peptides (CPPs) are promising tools for transducing presynthesized therapeutic molecules which possess low membrane permeability. The poor efficiency of cellular uptake and unexpected cellular localization are still the main obstacles to the development of drug delivery by using CPPs. In this study, we investigated the effect of a penetration enhancer, dimethylsulfoxide (DMSO), on the penetrating efficiency of a synthetic TAT peptide or the TAT fusion protein. FITC-labeled TAT and TAT-GFP were added to 10% DMSO or 100 mu M chloroquine pretreated cells, fluorescence uptake into culturing cells was observed using fluorescence microscopy, FACS or quantitatively analyzed by a fluorescence spectrum. 10% DMSO treatment markedly increased internalization of TAT into cells and appeared in a well-distributed pattern throughout the cytosol and nucleus without membrane perforating or detectable cytotoxicity, the enhancement effect by 10% DMSO was reduced by endocytosis inhibitors including ammonium chloride and sodium azide. 10% DMSO also enhanced TAT-Apoptin induced apoptosis of carcinoma cells. These findings implicated that DMSO can be a novel delivery enhancer appropriate for CPP penetration. (C) 2009 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000276766600009 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/69987]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Chang-Bai |
| 作者单位 | 1.Three Georges Univ, Inst Mol Biol, Yichang 443002, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hu,Zhong, Chun-Yan,Wu, Jiang-Feng,et al. Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide[J]. JOURNAL OF CONTROLLED RELEASE,2010,143(1):64-70. |
| APA | Wang, Hu,Zhong, Chun-Yan,Wu, Jiang-Feng,Huang, Yu-Bin,&Liu, Chang-Bai.(2010).Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide.JOURNAL OF CONTROLLED RELEASE,143(1),64-70. |
| MLA | Wang, Hu,et al."Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide".JOURNAL OF CONTROLLED RELEASE 143.1(2010):64-70. |
入库方式： OAI收割
来源：化学研究所
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