Carbazole tricationic salt: A novel potential two-photon fluorescent DNA probe for nucleic imaging of cells
文献类型：期刊论文
| 作者 | Zhao Ning1; Zhang YuanHong1; Liu Xin1; Yu XiaoQiang1; Ge MaoFa2 |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 55期号:32页码:3661-3667 |
| 关键词 | Two-photon Fluorescence Microscopy Two-photon Fluorescent Probe Dna Titration Dna Staining |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-010-4176-2 |
| 英文摘要 | A new carbazole tricationic salt, 4,4'-(1E,1'E)-2,2'-(9-(2-(1-(2-hydroxyethyl)pyridinium-4-yl)ethyl)-9H-carbazole-3,6-diyl) bis(ethane-2,1-diyl) bis(1-(2-hydroxyethyl)pyridinium) iodide (THEPC) was synthesized. Photophysical experiments have shown that THEPC has large two-photon excited fluorescence action cross-sections (33 GM in the presence of DNA), which ranks THEPC as a good biological fluorophore. The results from electronic absorption, circle dichroism and single-/two-photon fluorescence emission spectra suggest that THEPC can strongly bind to DNA, with an intrinsic binding constant of 5.79 x 10(6) L mol(-1). THEPC has better photostability under one- or two-photon excitation conditions. Finally, the staining photos from two-photon fluorescence microscopy (TPM) show that THEPC can exclusively label the nucleus with high contrast and without image distortion. These remarkable properties and optimized imaging ability make THEPC an attractive DNA probe in TPM. |
| 语种 | 英语 |
| WOS记录号 | WOS:000285026300006 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/70257]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yu XiaoQiang |
| 作者单位 | 1.Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Beijing Natl Lab Mol Sci BNLM, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao Ning,Zhang YuanHong,Liu Xin,et al. Carbazole tricationic salt: A novel potential two-photon fluorescent DNA probe for nucleic imaging of cells[J]. CHINESE SCIENCE BULLETIN,2010,55(32):3661-3667. |
| APA | Zhao Ning,Zhang YuanHong,Liu Xin,Yu XiaoQiang,&Ge MaoFa.(2010).Carbazole tricationic salt: A novel potential two-photon fluorescent DNA probe for nucleic imaging of cells.CHINESE SCIENCE BULLETIN,55(32),3661-3667. |
| MLA | Zhao Ning,et al."Carbazole tricationic salt: A novel potential two-photon fluorescent DNA probe for nucleic imaging of cells".CHINESE SCIENCE BULLETIN 55.32(2010):3661-3667. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。