A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles
文献类型：期刊论文
| 作者 | Liu, Xiaoyan1; Ren, Xiufang1; Deng, Xiaoyong2; Huo, Yinan1; Xie, Jiang4; Huang, Hai1; Jiao, Zheng2; Wu, Minghong2; Liu, Yuanfang2,3; Wen, Tieqiao1,5 |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 31期号:11页码:3063-3070 |
| 关键词 | Titanium Dioxide Nanoparticles Neural Stem Cells Differentiation Protein-protein Interaction Proteomics |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2009.12.054 |
| 英文摘要 | The effects of titanium dioxide (TiO(2)) nanoparticles (NPs) on the differentiation of neural stem cells are reported. Our findings indicate that TiO(2) NPs lead to a differentiational tendency towards neurons from neural stem cells, suggesting TiO(2) NPs might be a beneficial inducer for neuronal differentiation. To insight into the possible molecular mechanism of the neuronal differentiation, we conducted a protein-protein interaction network (PIN) analysis. To this end, a global mapping of target proteins induced by TiO(2) NPs was first made by a 2-dimensional electrophoresis analysis. Results showed that 9 proteins were significantly changed and then they were subjected to the mass spectrometric assay. All 9 identified proteins are involved in signal, molecular chaperones, cytoskeleton, and nucleoprotein. Further, based on our experimental data and DIP, IntAct-EBI, GRID database, a protein-protein interaction network was constructed, which provides highly integrated information exhibiting the protein-protein interaction. By analysis of the gene expression, the signal pathway involving Cx43 phosphotylation, which is negatively regulated by the protein kinase C epsilon (PKC epsilon), is demonstrated. It is inferred that PKCE plays a pivotal negative role in the neuronal differentiation of stem neural cells in response to the TiO(2) NPs exposure. (C) 2009 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000275946500013 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/70259]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wen, Tieqiao |
| 作者单位 | 1.Shanghai Univ, Sch Life Sci, Mol Neurobiol Lab, Shanghai 200444, Peoples R China 2.Shanghai Univ, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China 3.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 4.Shanghai Univ, Sch Comp Engn & Sci, Shanghai 200072, Peoples R China 5.Shanghai Univ, Inst Syst Biol, Shanghai 200444, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaoyan,Ren, Xiufang,Deng, Xiaoyong,et al. A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles[J]. BIOMATERIALS,2010,31(11):3063-3070. |
| APA | Liu, Xiaoyan.,Ren, Xiufang.,Deng, Xiaoyong.,Huo, Yinan.,Xie, Jiang.,...&Wen, Tieqiao.(2010).A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles.BIOMATERIALS,31(11),3063-3070. |
| MLA | Liu, Xiaoyan,et al."A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles".BIOMATERIALS 31.11(2010):3063-3070. |
入库方式： OAI收割
来源：化学研究所
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