Involvement of reactive oxygen species and calcium in photo-induced membrane damage in HeLa cells by a bis-methanophosphonate fullerene
文献类型：期刊论文
| 作者 | Qiao, Xinge1; Huang, Cheng1; Ying, Yabing1; Yang, Xinlin1; Liu, Yang2; Tian, Qiu2 |
| 刊名 | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
 |
| 出版日期 | 2010-03-08 |
| 卷号 | 98期号:3页码:193-198 |
| 关键词 | Fullerene Derivative Cell Membrane Damage Calcium Reactive Oxygen Species |
| ISSN号 | 1011-1344 |
| DOI | 10.1016/j.jphotobiol.2010.01.001 |
| 英文摘要 | Photo-excited bioactivities of fullerene derivatives are attracting much attention. In this report, a bismethanophosphonate fullerene (BMPF) and the other two fullerene derivatives, a bis-malonic acid fullerene (BMAF) and a fullerol were incubated with HeLa cells and irradiated with a green light emitted from a mercury lamp on a fluorescent microscopy. By using DNA fluorescent probe propidium iodide staining method, damage towards cell membrane could be detected when cells were treated by irradiation altogether with BMPF or BMAF at a low concentration (4 mu M), and the damage was dose-dependent. The activity of BMPF was much higher than that of BMAF, while fullerol had no effects under the same condition. It was also revealed that different kinds of reactive oxygen species (ROS) correlated to BMPF and BMAF. Additionally, presence of extracellular calcium could promote the activities of both derivatives, while removal of extracellular calcium could not abort their membrane-damaged activities. These results indicated that ROS and calcium were involved in the photosensitization of fullerene derivatives, and BMPF was a superior photosensitizer which would find potential application in biomedical field. (C) 2010 Elsevier By. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000276116600004 |
| 出版者 | ELSEVIER SCIENCE SA |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/70301]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, Xinlin |
| 作者单位 | 1.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiao, Xinge,Huang, Cheng,Ying, Yabing,et al. Involvement of reactive oxygen species and calcium in photo-induced membrane damage in HeLa cells by a bis-methanophosphonate fullerene[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,2010,98(3):193-198. |
| APA | Qiao, Xinge,Huang, Cheng,Ying, Yabing,Yang, Xinlin,Liu, Yang,&Tian, Qiu.(2010).Involvement of reactive oxygen species and calcium in photo-induced membrane damage in HeLa cells by a bis-methanophosphonate fullerene.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,98(3),193-198. |
| MLA | Qiao, Xinge,et al."Involvement of reactive oxygen species and calcium in photo-induced membrane damage in HeLa cells by a bis-methanophosphonate fullerene".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 98.3(2010):193-198. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。