Synthesis and characterization of multifunctional poly(glycidyl methacrylate) microspheres and their use in cell separation
文献类型：期刊论文
| 作者 | Dong, Xiaoqing1,2; Zheng, Yonghui1; Huang, Yubin1; Chen, Xuesi1; Jing, Xiabin1 |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2010-10-15 |
| 卷号 | 405期号:2页码:207-212 |
| 关键词 | Microspheres Fe(3)o(4) Nanoparticles Fluorescence Folic Acid Cell Detection And Separation |
| ISSN号 | 0003-2697 |
| DOI | 10.1016/j.ab.2010.06.022 |
| 英文摘要 | Multifunctional poly(glycidyl methacrylate) (PGMA) microspheres containing magnetic, fluorescent, and cancer cell-specific moieties were prepared in four steps: (i) preparation of parent PGMA microspheres by dispersion polymerization and their reaction with ethylenediamine to obtain amino groups, (ii) precipitation of iron ions (Fe(2+) and Fe(3+)) to form Fe(3)O(4) nanoparticles within the microspheres, (iii) consecutive reactions of folic acid with the amino groups on PGMA, and (iv) incorporation of fluorescein isothiocyanate into the microspheres. The microspheres were superparamagnetic, highly monodispersive, intensively fluorescent, and capable of recognizing and binding cancer cells that overexpress folic acid receptors. It was demonstrated that with these microspheres, HeLa cells could be captured from their suspension and easily moved in the direction of the externally applied magnetic field. (C) 2010 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000281296300009 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/70419]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jing, Xiabin |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Xiaoqing,Zheng, Yonghui,Huang, Yubin,et al. Synthesis and characterization of multifunctional poly(glycidyl methacrylate) microspheres and their use in cell separation[J]. ANALYTICAL BIOCHEMISTRY,2010,405(2):207-212. |
| APA | Dong, Xiaoqing,Zheng, Yonghui,Huang, Yubin,Chen, Xuesi,&Jing, Xiabin.(2010).Synthesis and characterization of multifunctional poly(glycidyl methacrylate) microspheres and their use in cell separation.ANALYTICAL BIOCHEMISTRY,405(2),207-212. |
| MLA | Dong, Xiaoqing,et al."Synthesis and characterization of multifunctional poly(glycidyl methacrylate) microspheres and their use in cell separation".ANALYTICAL BIOCHEMISTRY 405.2(2010):207-212. |
入库方式： OAI收割
来源：化学研究所
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