DNA Electrochemical Biosensor for Trace Hg2+ Detection
文献类型：期刊论文
| 作者 | Ge Fang1,2; Cao Rui-Guo1; Zhu Bin1; Li Jing-Jian1; Xu Dong-Sheng1 |
| 刊名 | ACTA PHYSICO-CHIMICA SINICA
 |
| 出版日期 | 2010-07-01 |
| 卷号 | 26期号:7页码:1779-1783 |
| 关键词 | Biosensor Hg2++++ Dna Conformational Change Differential Pulse Voltammetry Electrochemical Impedance Spectroscopy |
| ISSN号 | 1000-6818 |
| DOI | 10.3866/PKU.WHXB20100736 |
| 英文摘要 | In this paper we demonstrated a novel type of electrochemical Hg2+ biosensor based on a DNA-modified electrode. Ferrocenyl. modified T. rich DNA (DNA-Fc) molecules were synthesized for use as electrochemical probes. We then fixed these DNA-Fc probes onto a gold electrode surface by self. assembly. In the presence of Hg2+, the single strand DNA on the electrode surface turned to a thymine-Hg2+-thymine (T-Hg2+-T) hairpin structure. The ferrocenyl groups were kept away from the surface of the electrode, and this could be measured sensitively by differential pulse voltammetry (DPV). The results show a reduction peak of ferrocene at 0.26 V (vs saturated calomel electrode (SCE)) and the peak current of DPV decreased with increasing the concentration of Hg2+. The rate of current change is linear with regards to lgc(Hg2+) over a concentration range from 0.1 nmol . L-1 to 1 mu mol . L-1 and with a detection limit of 0.1 mol . L-1. A test for interference metal ions showed that this electrochemical biosensor based on a DNA modified electrode is highly sensitive and selective, and it can be widely used for trace Hg2+ detection. |
| 语种 | 英语 |
| WOS记录号 | WOS:000279776400008 |
| 出版者 | PEKING UNIV PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/70615]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li Jing-Jian |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Coll Sanming, Dept Chem & Biol Engn, Sanming 365004, Fujian Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge Fang,Cao Rui-Guo,Zhu Bin,et al. DNA Electrochemical Biosensor for Trace Hg2+ Detection[J]. ACTA PHYSICO-CHIMICA SINICA,2010,26(7):1779-1783. |
| APA | Ge Fang,Cao Rui-Guo,Zhu Bin,Li Jing-Jian,&Xu Dong-Sheng.(2010).DNA Electrochemical Biosensor for Trace Hg2+ Detection.ACTA PHYSICO-CHIMICA SINICA,26(7),1779-1783. |
| MLA | Ge Fang,et al."DNA Electrochemical Biosensor for Trace Hg2+ Detection".ACTA PHYSICO-CHIMICA SINICA 26.7(2010):1779-1783. |
入库方式： OAI收割
来源：化学研究所
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