Controlled Assembly of Protein in Glass Capillary
文献类型:期刊论文
作者 | Lin, Yuan2,3,4; Su, Zhaohui2; Balizan, Elizabeth3,4; Niu, Zhongwei1; Wang, Qian3,4 |
刊名 | LANGMUIR
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出版日期 | 2010-08-03 |
卷号 | 26期号:15页码:12803-12809 |
ISSN号 | 0743-7463 |
DOI | 10.1021/la1017888 |
英文摘要 | By means of a slow drying process and the control of surface charge characteristics, protein stripe patterns were readily prepared on the luminal surface of a capillary. We systematically studied the effects of surface properties, pH, and protein concentration on pattern formation using optical microscopy, atomic force microscopy, and quartz crystal microbalance measurement. By balancing these parameters, a broad selection of proteins could be assembled within a capillary with well-defined stripe patterns. Neutravidin, one of the model proteins, was specifically chosen to demonstrate the bioactivity retained through the assembly process by interaction with fluorescently labeled biotin motifs. This technique therefore offers a facile approach for patterning proteins and other biomacromolecules in capillary tubes. |
语种 | 英语 |
WOS记录号 | WOS:000280353800048 |
出版者 | AMER CHEMICAL SOC |
源URL | [http://ir.iccas.ac.cn/handle/121111/70639] ![]() |
专题 | 中国科学院化学研究所 |
通讯作者 | Niu, Zhongwei |
作者单位 | 1.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100910, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 3.Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA 4.Univ S Carolina, Nanoctr, Columbia, SC 29208 USA |
推荐引用方式 GB/T 7714 | Lin, Yuan,Su, Zhaohui,Balizan, Elizabeth,et al. Controlled Assembly of Protein in Glass Capillary[J]. LANGMUIR,2010,26(15):12803-12809. |
APA | Lin, Yuan,Su, Zhaohui,Balizan, Elizabeth,Niu, Zhongwei,&Wang, Qian.(2010).Controlled Assembly of Protein in Glass Capillary.LANGMUIR,26(15),12803-12809. |
MLA | Lin, Yuan,et al."Controlled Assembly of Protein in Glass Capillary".LANGMUIR 26.15(2010):12803-12809. |
入库方式: OAI收割
来源:化学研究所
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