Kinetic Study on the Reentrant Condensation of Oligonucleotide in Trivalent Salt Solution
文献类型：期刊论文
| 作者 | Zhou, Jihan; Ke, Fuyou1; Liang, Dehai |
| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY B
 |
| 出版日期 | 2010-11-04 |
| 卷号 | 114期号:43页码:13675-13680 |
| ISSN号 | 1520-6106 |
| DOI | 10.1021/jp1074187 |
| 英文摘要 | The reentrant condensation of 21-bp oligonucleotide in the presence of spermidine was investigated by laser light scattering and capillary electrophoresis. 21-bp oligonucleotide showed a bimodal distribution in 1 x TE buffer, with the slow mode being the characteristic diffusion of polyelectrolyte in solution without enough salt. At the fixed spermidine concentration, the reentry of oligonucleotide underwent aggregation, phase separation, and disassociation in sequence with time, and the kinetics was extremely slow. For example, it took more than 1200 h (50 days) for the reentry to complete at 21 mM spermidine. Higher spermidine concentration led to faster kinetics. After reentry, the slow mode disappeared, and the charges of oligonucleotide were at least partially neutralized. No prominent charge inversion was observed. The kinetics of oligonucleotide reentry in the presence of spermidine gained insight in the interactions of polyelectrolyte in aqueous solution. |
| 语种 | 英语 |
| WOS记录号 | WOS:000283519600008 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71037]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ke, Fuyou |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Key Lab Polymer Chem & Phys, Minist Educ, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Jihan,Ke, Fuyou,Liang, Dehai. Kinetic Study on the Reentrant Condensation of Oligonucleotide in Trivalent Salt Solution[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2010,114(43):13675-13680. |
| APA | Zhou, Jihan,Ke, Fuyou,&Liang, Dehai.(2010).Kinetic Study on the Reentrant Condensation of Oligonucleotide in Trivalent Salt Solution.JOURNAL OF PHYSICAL CHEMISTRY B,114(43),13675-13680. |
| MLA | Zhou, Jihan,et al."Kinetic Study on the Reentrant Condensation of Oligonucleotide in Trivalent Salt Solution".JOURNAL OF PHYSICAL CHEMISTRY B 114.43(2010):13675-13680. |
入库方式： OAI收割
来源：化学研究所
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