A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva
文献类型：期刊论文
| 作者 | Wang, Dan; Zhuo, Jun-Qiao; Zhao, Mei-Ping |
| 刊名 | TALANTA
 |
| 出版日期 | 2010-06-30 |
| 卷号 | 82期号:1页码:432-436 |
| 关键词 | Immunoglobulin a (Iga) Saliva Sample Competitive Enzyme-linked Immunosorbent Assay (Celisa) |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2010.04.040 |
| 英文摘要 | A simple competitive enzyme-linked immunosorbent assay (cELISA) was established for rapid measurement of secretory immunoglobulin A (sIgA) in saliva. The method was based on competitive reaction between the immobilized IgA and free IgA in the solution for the limited amount of horseradish peroxidase-conjugated rabbit anti-human IgA. In comparison with the conventionally used Sandwich ELISA, the cELISA is simpler, low-cost, and shows better reproducibility since it is not affected by the variation of capture antibodies from different batches. The assay time was also significantly reduced from more than 5 h to less than 3 h. Different curve-fitting models were compared, among which the fully specified logit-log model gave the best results. The linear working range and limit of detection were found to be 0.1-100 mu g mL(-1) and 0.05 mu g mL(-1), respectively. Matrix effects of saliva samples were investigated and a reasonable range of dilution factors were proposed. The developed method offers a very practical approach for high-throughput measurement of sIgA in saliva samples. (C) 2010 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000279488900063 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71059]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Mei-Ping |
| 作者单位 | Peking Univ, Coll Chem & Mol Engn, Minist Educ, Beijing Natl Lab Mol Sci,Key Lab Bioorgan Chem &, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Dan,Zhuo, Jun-Qiao,Zhao, Mei-Ping. A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva[J]. TALANTA,2010,82(1):432-436. |
| APA | Wang, Dan,Zhuo, Jun-Qiao,&Zhao, Mei-Ping.(2010).A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva.TALANTA,82(1),432-436. |
| MLA | Wang, Dan,et al."A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva".TALANTA 82.1(2010):432-436. |
入库方式： OAI收割
来源：化学研究所
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