Combination of DNA Ligase Reaction and Gold Nanoparticle-Quenched Fluorescent Oligonucleotides: A Simple and Efficient Approach for Fluorescent Assaying of Single-Nucleotide Polymorphisms
文献类型：期刊论文
| 作者 | Wang, Hao1,2; Li, Jishan1; Wang, Yongxiang1,2; Jin, Jiangyu1,2; Yang, Ronghua1; Wang, Kemin1; Tan, Weihong1 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2010-09-15 |
| 卷号 | 82期号:18页码:7684-7690 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac1015031 |
| 英文摘要 | A new fluorescent sensing approach for detection of single-nucleotide polymorphisms (SNPs) is proposed based on the ligase reaction and gold nanoparticle (AuNPs)-quenched fluorescent oligonucleotides. The design exploits the strong fluorescence quenching of AuNPs for organic dyes and the difference in noncovalent interactions of the nanoparticles with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA), where ssDNA can be adsorbed onto the surface of AuNPs while dsDNA cannot be. In the assay, two half primer DNA probes, one being labeled with a dye and the other being phosphorylated, were first incubated with a target DNA template. In the presence of DNA ligase, the two captured ssDNAs are linked for the perfectly matched DNA target to form a stable duplex, but the duplex could not be formed by the single-base mismatched DNA template. After addition of AuNPs, the fluorescence of dye-tagged DNA probe will be efficiently quenched unless the perfectly matched DNA target is present. To demonstrate the feasibility of this design, the performance of SNP detection using two different DNA ligases, T4 DNA ligase and Eschcrichia coli DNA ligase, were investigated. In the case of T4 DNA ligase, the signal enhancement of the dye-tagged DNA for perfectly matched DNA target is 4.6-fold higher than that for the single-base mismatched DNA. While in the presence of E. coli DNA ligase, the value raises to be 30.2, suggesting excellent capability for SNP discrimination. |
| 语种 | 英语 |
| WOS记录号 | WOS:000281710900023 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71127]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, Ronghua |
| 作者单位 | 1.Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Coll Biol, Changsha 410082, Hunan, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hao,Li, Jishan,Wang, Yongxiang,et al. Combination of DNA Ligase Reaction and Gold Nanoparticle-Quenched Fluorescent Oligonucleotides: A Simple and Efficient Approach for Fluorescent Assaying of Single-Nucleotide Polymorphisms[J]. ANALYTICAL CHEMISTRY,2010,82(18):7684-7690. |
| APA | Wang, Hao.,Li, Jishan.,Wang, Yongxiang.,Jin, Jiangyu.,Yang, Ronghua.,...&Tan, Weihong.(2010).Combination of DNA Ligase Reaction and Gold Nanoparticle-Quenched Fluorescent Oligonucleotides: A Simple and Efficient Approach for Fluorescent Assaying of Single-Nucleotide Polymorphisms.ANALYTICAL CHEMISTRY,82(18),7684-7690. |
| MLA | Wang, Hao,et al."Combination of DNA Ligase Reaction and Gold Nanoparticle-Quenched Fluorescent Oligonucleotides: A Simple and Efficient Approach for Fluorescent Assaying of Single-Nucleotide Polymorphisms".ANALYTICAL CHEMISTRY 82.18(2010):7684-7690. |
入库方式： OAI收割
来源：化学研究所
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