Water-Soluble Conjugated Polymers for Amplified Fluorescence Detection of Template-Independent DNA Elongation Catalyzed by Polymerase
文献类型：期刊论文
| 作者 | He, Fang1; Liu, Libing2; Li, Lidong1 |
| 刊名 | ADVANCED FUNCTIONAL MATERIALS
 |
| 出版日期 | 2011-08-23 |
| 卷号 | 21期号:16页码:3143-3149 |
| ISSN号 | 1616-301X |
| DOI | 10.1002/adfm.201100216 |
| 英文摘要 | A series of water-soluble polyfluorene derivatives containing diketopyrrolopyrrole derivative units are synthesized and characterized. These copolymers, poly[9,9'-bis(6 ''-N,N,N-trimethyl ammonium) hexylfluorene-co-alt-2,5-bis (6 ''-N,N,N-trimethylammonium) hexylpyrrolo[3,4-c]pyrrole-1,4(2H, 5H)-dione] (PFDPN), demonstrate intramolecular energy transfer from the fluorene units to the diketopyrrolopyrrole derivative units, and show red-shifted emissions in aqueous solution. The PFDPN polymers can combine with Cy5-labeled ssDNA by electrostatic interactions and efficiently amplify the fluorescence signal of red Cy5 dye through fluorescence resonance energy transfer. Moreover, based on DNA replacement method, this amplification system can be used to monitor the template-independent DNA elongation process catalyzed by terminal deoxynucleotidyl transferase. |
| 语种 | 英语 |
| WOS记录号 | WOS:000294166200016 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71277]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | He, Fang |
| 作者单位 | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Fang,Liu, Libing,Li, Lidong. Water-Soluble Conjugated Polymers for Amplified Fluorescence Detection of Template-Independent DNA Elongation Catalyzed by Polymerase[J]. ADVANCED FUNCTIONAL MATERIALS,2011,21(16):3143-3149. |
| APA | He, Fang,Liu, Libing,&Li, Lidong.(2011).Water-Soluble Conjugated Polymers for Amplified Fluorescence Detection of Template-Independent DNA Elongation Catalyzed by Polymerase.ADVANCED FUNCTIONAL MATERIALS,21(16),3143-3149. |
| MLA | He, Fang,et al."Water-Soluble Conjugated Polymers for Amplified Fluorescence Detection of Template-Independent DNA Elongation Catalyzed by Polymerase".ADVANCED FUNCTIONAL MATERIALS 21.16(2011):3143-3149. |
入库方式： OAI收割
来源：化学研究所
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