Fluorescence study of interaction between an anionic conjugated polyelectrolyte and bovine serum albumin
文献类型：期刊论文
| 作者 | Wang, Yani1; Dong, Jie1; Liu, Chuanzhen2; Bao, Biqing3; Wang, Lianhui3; Zhan, Xiaowei4; Yang, Huai1; Wang, Guojie1 |
| 刊名 | POLYMER BULLETIN
 |
| 出版日期 | 2011-12-01 |
| 卷号 | 67期号:9页码:1907-1915 |
| 关键词 | Fluorescence Biosensor Conjugated Polyelectrolyte Protein Probe |
| ISSN号 | 0170-0839 |
| DOI | 10.1007/s00289-011-0577-x |
| 英文摘要 | The interaction between an anionic conjugated polyelectrolyte, poly[9,9-bis(3'-butyrate)fluoren-2,7-yl] sodium (BBS-PF), and bovine serum albumin was investigated by fluorescence spectroscopy. The emission of BBS-PF was effectively quenched by BSA with a quenching constant K (SV) of 3.1 x 10(7) L/mol when BSA was at nanomolar concentrations, but the emission increased when the concentration of BSA was at micromolar level. The excitation band of BBS-PF blue-shifted when the emission was quenched where the negatively charged BSA induced the aggregation of BBS-PF, yet the excitation band of BBS-PF red-shifted when the emission increased where the BSA acted as a surfactant and formed hydrophobic interaction with BBS-PF. BBS-PF could also quench BSA through energy transfer by resonance with a quenching constant K (SV) of 1.1 x 10(6) L/mol. The emission band changes of BSA reflected the conformation transitions of BSA from class II to class I and the binding of BBS-PF with BSA made the BSA more folded. |
| 语种 | 英语 |
| WOS记录号 | WOS:000296732500015 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71373]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Guojie |
| 作者单位 | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Beijing BOE Display Technol Co Ltd, Beijing 100176, Peoples R China 3.Fudan Univ, Adv Mat Lab, Shanghai 200433, Peoples R China 4.Chinese Acad Sci, Inst Chem, CAS Key Lab Organ Solids, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yani,Dong, Jie,Liu, Chuanzhen,et al. Fluorescence study of interaction between an anionic conjugated polyelectrolyte and bovine serum albumin[J]. POLYMER BULLETIN,2011,67(9):1907-1915. |
| APA | Wang, Yani.,Dong, Jie.,Liu, Chuanzhen.,Bao, Biqing.,Wang, Lianhui.,...&Wang, Guojie.(2011).Fluorescence study of interaction between an anionic conjugated polyelectrolyte and bovine serum albumin.POLYMER BULLETIN,67(9),1907-1915. |
| MLA | Wang, Yani,et al."Fluorescence study of interaction between an anionic conjugated polyelectrolyte and bovine serum albumin".POLYMER BULLETIN 67.9(2011):1907-1915. |
入库方式： OAI收割
来源：化学研究所
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