中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 化学研究所
  3. 中国科学院化学研究所
Engineering a zinc binding site into the de novo designed protein DS119 with a beta alpha beta structure

文献类型：期刊论文

作者Zhu, Cheng1; Zhang, Changsheng1; Liang, Huanhuan1; Lai, Luhua1,2
刊名PROTEIN & CELL
出版日期2011-12-01
卷号2期号:12页码:1006-1013
关键词Beta Alpha Beta De Novo Design Folding Zinc-binding
ISSN号1674-800X
DOI10.1007/s13238-011-1121-3
英文摘要Functional proteins designed de novo have potential application in chemical engineering, agriculture and healthcare. Metal binding sites are commonly used to incorporate functions. Based on a de novo designed protein DS119 with a beta alpha beta structure, we have computationally engineered zinc binding sites into it using a home-made searching program. Seven out of the eight designed sequences tested were shown to bind Zn2+ with micromolar affinity, and one of them bound Zn2+ with 1:1 stoichiometry. This is the first time that metalloproteins with an alpha, beta mixed structure have been designed from scratch.
语种英语
WOS记录号WOS:000310522900009
出版者HIGHER EDUCATION PRESS
源URL[http://ir.iccas.ac.cn/handle/121111/71399]  
专题中国科学院化学研究所
通讯作者Lai, Luhua
作者单位1.Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
2.Peking Univ, Ctr Theoret Biol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714		 Zhu, Cheng,Zhang, Changsheng,Liang, Huanhuan,et al. Engineering a zinc binding site into the de novo designed protein DS119 with a beta alpha beta structure[J]. PROTEIN & CELL,2011,2(12):1006-1013.
APA Zhu, Cheng,Zhang, Changsheng,Liang, Huanhuan,&Lai, Luhua.(2011).Engineering a zinc binding site into the de novo designed protein DS119 with a beta alpha beta structure.PROTEIN & CELL,2(12),1006-1013.
MLA Zhu, Cheng,et al."Engineering a zinc binding site into the de novo designed protein DS119 with a beta alpha beta structure".PROTEIN & CELL 2.12(2011):1006-1013.

入库方式： OAI收割

来源：化学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。