Highly sensitive detection of the hepatotoxin microcystin-LR by surface modification and bio-nanotechnology
文献类型：期刊论文
| 作者 | Han, Jianhua; Zhang, Jianping; Xia, Yuetong; Jiang, Long |
| 刊名 | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
 |
| 出版日期 | 2011-11-05 |
| 卷号 | 391期号:1-3页码:184-189 |
| 关键词 | Quartz Crystal Microbalance Microcystin-lr Immunoassay Gold Nanoparticles |
| ISSN号 | 0927-7757 |
| DOI | 10.1016/j.colsurfa.2011.01.043 |
| 英文摘要 | A highly sensitive, portable method of detecting the hepatotoxin microcystin-LR (MC-LR) has been developed by a surface modification and gold nanoparticle (AuNP) technology. By using this technology a detection sensitivity of 0.1 pg mu L-1 MC-LR can be reached which meets the standard of World Health Organization (WHO) requirements for the drinking water (1 mu g mu L-1 MC-LR) and compatible with those of conventional techniques, such as high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA). Our method includes an inexpensive sensor, composed of a quartz crystal microbalance (QCM) along with gold nanoparticles (AuNPs), and a sandwiched immunoassay for rapid and in situ detection of MC-LR. The volume of analyte for once detection was generally less than 50 mu L The method specifically involves self-assembled monolayer formation on the QCM gold electrodes, immobilisation of MC-LR antibodies and AuNP signal amplification. It has been found that 30 nm is the optimal size for AuNPs which could effectively amplify the signals of MC. All the process was easily and promptly operated. Thus, a fast and convenient sandwiched immunoassay sensor has been established. (C) 2011 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000299068100026 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71469]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jiang, Long |
| 作者单位 | Chinese Acad Sci, Colloids & Interfaces Lab, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Jianhua,Zhang, Jianping,Xia, Yuetong,et al. Highly sensitive detection of the hepatotoxin microcystin-LR by surface modification and bio-nanotechnology[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2011,391(1-3):184-189. |
| APA | Han, Jianhua,Zhang, Jianping,Xia, Yuetong,&Jiang, Long.(2011).Highly sensitive detection of the hepatotoxin microcystin-LR by surface modification and bio-nanotechnology.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,391(1-3),184-189. |
| MLA | Han, Jianhua,et al."Highly sensitive detection of the hepatotoxin microcystin-LR by surface modification and bio-nanotechnology".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 391.1-3(2011):184-189. |
入库方式： OAI收割
来源：化学研究所
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