TGF-beta signalling is mediated by two autonomously functioning T beta RI:T beta RII pairs
文献类型：期刊论文
| 作者 | Huang, Tao1; David, Laurent2; Mendoza, Valentin3; Yang, Yong4; Villarreal, Maria1; De, Keya5; Sun, LuZhe5; Fang, Xiaohong4; Lopez-Casillas, Fernando3; Wrana, Jeffrey L.2,6 |
| 刊名 | EMBO JOURNAL
 |
| 出版日期 | 2011-04-06 |
| 卷号 | 30期号:7页码:1263-1276 |
| 关键词 | Signal Transduction Signalling t Beta Ri t Beta Rii Tgf-beta |
| ISSN号 | 0261-4189 |
| DOI | 10.1038/emboj.2011.54 |
| 英文摘要 | Transforming growth factor (TGF)-beta s are dimeric polypeptides that have vital roles in regulating cell growth and differentiation. They signal by assembling a receptor heterotetramer composed of two T beta RI:T beta RII heterodimers. To investigate whether the two heterodimers bind and signal autonomously, one of the TGF-beta protomers was substituted to block receptor binding. The substituted dimer, TGF-beta 3 WD, bound the TbRII extracellular domain and recruited the TbRI with affinities indistinguishable from TGF-beta 3, but with one-half the stoichiometry. TGF-beta 3 WD was further shown to retain one-quarter to one-half the signalling activity of TGF-beta 3 in three established assays for TGF-beta function. Single-molecule fluorescence imaging with GFP-tagged receptors demonstrated a measurable increase in the proportion of TbRI and TbRII dimers upon treatment with TGF-beta 3, but not with TGF-beta 3 WD. These results provide evidence that the two T beta RI:T beta RII heterodimers bind and signal in an autonomous manner. They further underscore how the TGF-beta s diverged from the bone morphogenetic proteins, the ancestral ligands of the TGF-beta superfamily that signal through a RI:RII:RII heterotrimer. The EMBO Journal (2011) 30, 1263-1276. doi: 10.1038/emboj.2011.54; Published online 18 March 2011 |
| 语种 | 英语 |
| WOS记录号 | WOS:000290305200011 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71491]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Hinck, Andrew P. |
| 作者单位 | 1.Univ Texas Hlth Sci Ctr San Antonio, Dept Biochem, San Antonio, TX 78229 USA 2.Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Ctr Syst Biol, Toronto, ON M5G 1X5, Canada 3.Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Ciudad De Mexico, Mexico 4.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100080, Peoples R China 5.Univ Texas Hlth Sci Ctr San Antonio, Dept Cellular & Struct Biol, San Antonio, TX 78229 USA 6.Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A1, Canada |
| 推荐引用方式 GB/T 7714 | Huang, Tao,David, Laurent,Mendoza, Valentin,et al. TGF-beta signalling is mediated by two autonomously functioning T beta RI:T beta RII pairs[J]. EMBO JOURNAL,2011,30(7):1263-1276. |
| APA | Huang, Tao.,David, Laurent.,Mendoza, Valentin.,Yang, Yong.,Villarreal, Maria.,...&Hinck, Andrew P..(2011).TGF-beta signalling is mediated by two autonomously functioning T beta RI:T beta RII pairs.EMBO JOURNAL,30(7),1263-1276. |
| MLA | Huang, Tao,et al."TGF-beta signalling is mediated by two autonomously functioning T beta RI:T beta RII pairs".EMBO JOURNAL 30.7(2011):1263-1276. |
入库方式： OAI收割
来源：化学研究所
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