Colorimetric detection of glucose and an assay for acetylcholinesterase with amine-terminated polydiacetylene vesicles
文献类型：期刊论文
| 作者 | Xue WangXin; Zhang DeQing; Zhang GuanXin; Zhu DaoBen |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 56期号:18页码:1877-1883 |
| 关键词 | Polydiacetylene Vesicles Glucose Glucose Oxidase Acetylcholinesterase |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-011-4505-0 |
| 英文摘要 | The colorimetric response of amine-terminated polydiacetylene (PDA) vesicles was initially demonstrated by varying the pH of the solution. Convenient colorimetric methods to detect glucose and acetylcholinesterase (AChE) activity were successfully established using amine-terminated PDA vesicles by taking advantage of the following features: (1) the amine-terminated PDA vesicles undergo a colorimetric transition as the pH of the solution changes; (2) glucose can be oxidized to gluconic acid in the presence of glucose oxidase; and (3) AChE catalyzes the hydrolysis of acetylcholine to acetic acid. The visual detection of glucose levels and AChE activity showed good selectivity and acceptable sensitivity. The detection limit of glucose was similar to 2.5 mu mol/L and the level of AChE activity was assayed as low as 10.0 mU/mL. Moreover, the amine-terminated PDA vesicles can be used for screening the activity of inhibitors against AChE. |
| 语种 | 英语 |
| WOS记录号 | WOS:000291259500002 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71537]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang DeQing |
| 作者单位 | Chinese Acad Sci, Inst Chem, Organ Solids Lab, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue WangXin,Zhang DeQing,Zhang GuanXin,et al. Colorimetric detection of glucose and an assay for acetylcholinesterase with amine-terminated polydiacetylene vesicles[J]. CHINESE SCIENCE BULLETIN,2011,56(18):1877-1883. |
| APA | Xue WangXin,Zhang DeQing,Zhang GuanXin,&Zhu DaoBen.(2011).Colorimetric detection of glucose and an assay for acetylcholinesterase with amine-terminated polydiacetylene vesicles.CHINESE SCIENCE BULLETIN,56(18),1877-1883. |
| MLA | Xue WangXin,et al."Colorimetric detection of glucose and an assay for acetylcholinesterase with amine-terminated polydiacetylene vesicles".CHINESE SCIENCE BULLETIN 56.18(2011):1877-1883. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。