DNA template-synthesized silver nanoparticles: A new platform for high-performance fluorescent biosensing of biothiols
文献类型：期刊论文
| 作者 | Jin JianYu1; Ouyang XiangYuan2; Li JiShan2; Jiang JianHui2; Wang Hao1; Wang YongXiang1; Yang RongHua1,2 |
| 刊名 | SCIENCE CHINA-CHEMISTRY
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 54期号:8页码:1266-1272 |
| 关键词 | Silver Nanoparticles Dna Template-synthesized Thiols Fluorescent Sensing |
| ISSN号 | 1674-7291 |
| DOI | 10.1007/s11426-011-4320-0 |
| 英文摘要 | To develop the high-performance fluorescent bio-sensors, the metal nanoparticles were employed as nanoquenchers and attracted reasonable attention in the design of fluorescent biosensors. In this work, silver nanoparticles (AgNPs) were obtained via reduction of Ag(+) on FAM-labeled DNA template. For the tight binding between AgNPs and DNA, the template-synthesized AgNPs turned out high quenching efficiency and could be applied as super nanoquenchers to establish the biosensing platform for fluorescent detection. As an example, the template-synthesized DNA-AgNPs conjugates were employed in sensing thiols. By forming S-Ag bonds, thiols interact intensely with AgNPs and replace the FAM-labeled DNA off from the surface of AgNPs, resulting in a fluorescence enhancement. Besides the advantages of lower background and higher signal-to-background ratio (S/B), the conjugates present better stability, making them applicable in complicated biological fluids. To further evidence the feasibility of sensing thiols in real samples, the thiols in human urine were detected. The total amount of free thiols found in human urine was ranging from 229 mu M to 302 mu M with the proposed sensor. To conclude the reliability, low content of Cys was added and the recovery was 98%-103%. |
| 语种 | 英语 |
| WOS记录号 | WOS:000294062100012 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71565]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang RongHua |
| 作者单位 | 1.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Ctr Biomed Engn, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin JianYu,Ouyang XiangYuan,Li JiShan,et al. DNA template-synthesized silver nanoparticles: A new platform for high-performance fluorescent biosensing of biothiols[J]. SCIENCE CHINA-CHEMISTRY,2011,54(8):1266-1272. |
| APA | Jin JianYu.,Ouyang XiangYuan.,Li JiShan.,Jiang JianHui.,Wang Hao.,...&Yang RongHua.(2011).DNA template-synthesized silver nanoparticles: A new platform for high-performance fluorescent biosensing of biothiols.SCIENCE CHINA-CHEMISTRY,54(8),1266-1272. |
| MLA | Jin JianYu,et al."DNA template-synthesized silver nanoparticles: A new platform for high-performance fluorescent biosensing of biothiols".SCIENCE CHINA-CHEMISTRY 54.8(2011):1266-1272. |
入库方式： OAI收割
来源：化学研究所
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