PEG MODIFICATION AND PROTEIN CONJUGATION BY VIRTUE OF 4-AZIDOBENZOIC ACID AS A PHOTOAFFINITY MOLECULE
文献类型：期刊论文
| 作者 | Wei Jizheng1,2; Yang Lixin1; Hu Xiuli1; Huang Yubin1; Chen Xuesi1; Jing Xiabin1 |
| 刊名 | ACTA POLYMERICA SINICA
 |
| 出版日期 | 2011-11-20 |
| 期号 | 11页码:1341-1348 |
| 关键词 | Photoaffinity Labeling Photoactive Polymer Protein Immobilization Peg Modification |
| ISSN号 | 1000-3304 |
| DOI | 10.3724/SP.J.1105.2011.11080 |
| 英文摘要 | A photoaffinity labeling molecule, 4-azidobenzoic acid, was synthesized and introduced into P(LA-co-DHP) to form a photoactive copolymer P(LA-co-DAP). Tests showed that proteins could be immobilized on the polymer fibers of P( LA-co-DAP) within 5 min through a photoaffinity reaction. This suggested potential applications of photoaffinity coupling in protein immobilization and protein detection. Furthermore,PEG and 4-azidobenzoic acid were reacted in solution under UV irradiation to achieve the modification on PEG chains. The PEG molecules assembled on gold surface were reacted also with 4-azidobenzoic acid under UV irradiation. BSA adsorption test by FT-SPR showed that the 4-azidobenzoic acid moieties were successfully attached to the PEG segments so that the protein adsorption ability of the PEG surface was changed. In this way, functional groups like -PhCOOH were introduced onto PEG chains in a convenient and quick manner. This would offer a new method to achieve the modification of the polymers lacking of functional groups. |
| 语种 | 英语 |
| WOS记录号 | WOS:000297825700017 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71581]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wei Jizheng |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei Jizheng,Yang Lixin,Hu Xiuli,et al. PEG MODIFICATION AND PROTEIN CONJUGATION BY VIRTUE OF 4-AZIDOBENZOIC ACID AS A PHOTOAFFINITY MOLECULE[J]. ACTA POLYMERICA SINICA,2011(11):1341-1348. |
| APA | Wei Jizheng,Yang Lixin,Hu Xiuli,Huang Yubin,Chen Xuesi,&Jing Xiabin.(2011).PEG MODIFICATION AND PROTEIN CONJUGATION BY VIRTUE OF 4-AZIDOBENZOIC ACID AS A PHOTOAFFINITY MOLECULE.ACTA POLYMERICA SINICA(11),1341-1348. |
| MLA | Wei Jizheng,et al."PEG MODIFICATION AND PROTEIN CONJUGATION BY VIRTUE OF 4-AZIDOBENZOIC ACID AS A PHOTOAFFINITY MOLECULE".ACTA POLYMERICA SINICA .11(2011):1341-1348. |
入库方式： OAI收割
来源：化学研究所
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