Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution
文献类型：期刊论文
| 作者 | Ge, Jing; Liu, Zhaofang; Zhi, Zeyong; Zhao, Xin Sheng1 |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 413期号:1页码:75-77 |
| ISSN号 | 0003-2697 |
| DOI | 10.1016/j.ab.2011.01.032 |
| 英文摘要 | It has been a long-standing challenge in bioassay using aptamers and gold nanoparticles to detect disease-related proteins and other substance directly in complex biological samples such as serum. Here we propose a progressive dilution (PD) method to achieve simultaneous qualitative and quantitative analysis of proteins in blood serum without pretreatment of the sample. Above the detection limit, PD has unlimited dynamic range. We demonstrate the PD strategy through the detection of thrombin in fetal bovine serum using the quenching of fluorescence by gold nanoparticles. (C) 2011 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000289607900015 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71691]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Xin Sheng |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Jing,Liu, Zhaofang,Zhi, Zeyong,et al. Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution[J]. ANALYTICAL BIOCHEMISTRY,2011,413(1):75-77. |
| APA | Ge, Jing,Liu, Zhaofang,Zhi, Zeyong,&Zhao, Xin Sheng.(2011).Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution.ANALYTICAL BIOCHEMISTRY,413(1),75-77. |
| MLA | Ge, Jing,et al."Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution".ANALYTICAL BIOCHEMISTRY 413.1(2011):75-77. |
入库方式： OAI收割
来源：化学研究所
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