Highly effective protein detection for avidin-biotin system based on colloidal photonic crystals enhanced fluoroimmunoassay
文献类型：期刊论文
| 作者 | Shen, Weizhi; Li, Mingzhu; Xu, Liang; Wang, Shutao; Jiang, Lei; Song, Yanlin; Zhu, Daoben |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2011-01-15 |
| 卷号 | 26期号:5页码:2165-2170 |
| 关键词 | Photonic Crystal Fluoroimmunoassay Protein Detection Fluorescence Enhancement Background Interference |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2010.09.026 |
| 英文摘要 | There has been immense interest in both instruments and methods to enhance fluorescence signal and achieve highly sensitive fluoroimmunoassay (FIA). In this paper, we present a facile, low-cost and general method of biotinylated colloidal photonic crystal (PC) to improve the FIA of avidin (avidin FIA). The fluorescence signal intensity of the avidin FIA on the colloidal PC can be enhanced over two orders of magnitude relative to the control sample, attributed to the large surface area, resonance field and coherent scattering effect of the colloidal PC. The detection limit is shrunk to 1/69 of that of the control sample. Furthermore, the signal to interference ratio (S/I ratio) is increased because the band-edge induced fluorescence enhancement is wavelength-selective. The interference fluorescence does not go up proportionally while the signal is significantly enhanced by the colloidal PCs. It is believed that the colloidal PC modified with biotin can act as an effective material for a general and sensitive fluoroimmunoassay. (C) 2010 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000286904400060 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71791]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Mingzhu |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Organ Solids,Lab New Mat, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Weizhi,Li, Mingzhu,Xu, Liang,et al. Highly effective protein detection for avidin-biotin system based on colloidal photonic crystals enhanced fluoroimmunoassay[J]. BIOSENSORS & BIOELECTRONICS,2011,26(5):2165-2170. |
| APA | Shen, Weizhi.,Li, Mingzhu.,Xu, Liang.,Wang, Shutao.,Jiang, Lei.,...&Zhu, Daoben.(2011).Highly effective protein detection for avidin-biotin system based on colloidal photonic crystals enhanced fluoroimmunoassay.BIOSENSORS & BIOELECTRONICS,26(5),2165-2170. |
| MLA | Shen, Weizhi,et al."Highly effective protein detection for avidin-biotin system based on colloidal photonic crystals enhanced fluoroimmunoassay".BIOSENSORS & BIOELECTRONICS 26.5(2011):2165-2170. |
入库方式： OAI收割
来源：化学研究所
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