A New Fluorescence Turn-on Assay for Trypsin and Inhibitor Screening Based on Graphene Oxide
文献类型：期刊论文
| 作者 | Gu, Xinggui1,2; Yang, Ge1,2; Zhang, Guanxin1; Zhang, Deqing1; Zhu, Daoben1 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 3期号:4页码:1175-1179 |
| 关键词 | Graphene Oxide Fluorescein-labled Peptide Trypsin Assay Inhibitor Screening Fluorescence Quenching Fluorescence Turn-on |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/am2000104 |
| 英文摘要 | In this paper, we describe a new continuous fluorescence turn-on method for trypsin assay and inhibitor screening in situ. This assay is designed based on the following assumptions: (1) It is expected that the fluorescein-labeled peptide composed of six arginine residues (Arg(6)-FAM) with positive charges will interact with the negatively charged edge of water-soluble graphene oxide (GO) because of electrostatic interactions to form a GO/Arg(6)-FAM complex. As a result, the fluorescence of fluorescein will be quenched because of the energy transfer from fluorescein to GO. (2) Arg(6)-FAM can be hydrolyzed into small fragments in the presence of trypsin, and accordingly, the GO/Arg(6)-FAM complex will be dissociated, gradually leading to fluorescence recovery for the solution. In this way, the trypsin activity can be easily assayed with the ensemble of Arg(6)-FAM and GO. Additionally, the ensemble can be employed for screening of the inhibitors of trypsin. |
| 语种 | 英语 |
| WOS记录号 | WOS:000289762400035 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71903]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Guanxin |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Organ Solids Lab, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Xinggui,Yang, Ge,Zhang, Guanxin,et al. A New Fluorescence Turn-on Assay for Trypsin and Inhibitor Screening Based on Graphene Oxide[J]. ACS APPLIED MATERIALS & INTERFACES,2011,3(4):1175-1179. |
| APA | Gu, Xinggui,Yang, Ge,Zhang, Guanxin,Zhang, Deqing,&Zhu, Daoben.(2011).A New Fluorescence Turn-on Assay for Trypsin and Inhibitor Screening Based on Graphene Oxide.ACS APPLIED MATERIALS & INTERFACES,3(4),1175-1179. |
| MLA | Gu, Xinggui,et al."A New Fluorescence Turn-on Assay for Trypsin and Inhibitor Screening Based on Graphene Oxide".ACS APPLIED MATERIALS & INTERFACES 3.4(2011):1175-1179. |
入库方式： OAI收割
来源：化学研究所
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